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Modified Organism
AAT-7Ø9AA-4 - Pod Borer-resistant cowpea
Record information and status
Record ID
114444
Status
Published
Date of creation
2019-02-26 19:24 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-02-26 19:24 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Pod Borer-resistant cowpea
Transformation event
AAT709A
Unique identifier
AAT-7Ø9AA-4
Developer(s)
AATF and Institute of Agricultural Research
African Agricultural Technology Foundation
ARCN Annex No. 3 Ibrahim Idris Street, Jabi
Abuja, F.C.T
Nigeria
Phone:254-204223700
Fax:254-204223701
Email:aatf@aatf-africa.org
Description
Cowpea cultivar IT86D-1010 was modified via Agrobacterium-mediated transformation for the expression a truncated version of the Cry1Ab protein from Bacillus thuringiensis subs. kurstaki strain HD-1 for resistance against Pod Borer (Maruca vitrata).
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Vigna unguiculata - Cowpea, Black eyed pea
Characteristics of the transformation process
Vector
pMB4 derived from pART27
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
SCSV1 promoter
0.52 Kb
 
 
Neomycin Phosphotransferase II
0.18 Kb
 
 
Catalase 1 intron
0.19 Kb
 
 
Neomycin Phosphotransferase II
0.62 Kb
 
 
pMB4 intervening sequence
0.18 Kb
 
 
SCSV3 terminator
0.14 Kb
 
 
rbcS Promoter
1.73 Kb
 
 
Cry1Ab
1.85 Kb
 
 
rbcS Terminator
0.37 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Transcription of cry1Ab occurs from the Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase (Rubisco) small subunit promoter, which also contains the 5' untranslated region. Transcription terminates at the Nicotiana tabacum ribulose-1,5-bisphosphate carboxylase small subunit terminator. The nucleotide sequence of cry1Ab was codon optimized for plant expression and encodes a 615-amino acid protein (68.9 kDa), corresponding to the trypsin-resistant, insecticidally active core protein, following cleavage of the 1155-amino acid native Cry1Ab protoxin.

For selection during cloning, a kanamycin resistance gene cassette was also integrated into the genome. Transcription of the neomycin phosphotranferase II (nphII) gene starts at the subterranean clover stunt virus (SCSV) 1 promoter. Two segments of nptII are transcribed, with a catalase-1 intron from Ricinus communis between the two segments and an intervening sequence from the MB4 plasmid following the second segment. Transcription stops at the SCSV3 terminator.
LMO characteristics
Modified traits
Common use(s)
  • Food

Records referencing this document (2)
IDDescription
2record(s) found
Modified Organism2 records