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Modified Organism
Cisgenic barley with improved phytase activity
Record information and status
Record ID
114555
Status
Published
Date of creation
2019-03-21 12:59 UTC (lbo@lbst.dk)
Date of publication
2019-03-21 15:19 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Cisgenic barley with improved phytase activity
Transformation event
PaPhy07
Developer(s)
Ms. Inger Bæksted Holme
Senior scientist
Research Center Flakkebjerg
Aarhus University (AU)
Forsøgsvej 1
Slagelse
Denmark, 4200
Phone:+4587158238
Email:inger.holme@mbg.au.dk
Description
The modified barley has increased phytase activity due to the presence of an additional copy of the phytase gene (cisgenic). The increase phytase activity increases the bioavailability of phosphorous within the mature barley grains.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Hordeum vulgare - Barley, HORVU
Point of collection or acquisition of the recipient organism
Cv. Golden Promise
Related LMOs
Barley modified to produce phytase
Dr. Ludmila Ohnoutková Changes in quality and/or metabolite content Phytate degradation Resistance to herbicides - Glufosinate Selectable marker genes and reporter genes
Characteristics of the transformation process
Vector
pTiT37
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Barley Phytase Promoter
2.18 Kb
 
 
Barley Phytase
2.27 Kb
 
 
Barley Phytase Terminator
0.76 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The barley (Hordeum vulgare) Phytase gene (HvPAPhy_a) is under the control of the native promoter. Transcription terminates at the native terminator. The additional copy of the phytase gene leads to an increase in the phytase activity in the mature grains.

Note:
- No selection genes or vector-backbone sequences were present in the modified barley line.
- The modified barley line had not previously been modified.
LMO characteristics
Modified traits
Other gene(s) whose expression was affected by the transformation
Barley Phytase - Hordeum vulgare - Barley, HORVU
Changes in quality and/or metabolite content - Protein and amino acids, Vitamins
How the expression of the gene(s) was affected
An additional copy of HvPAPhy_a leads to an increased expression and activity of HvPAPHY_A in the mature grains.
Common use(s)
  • Feed
Additional Information
Additional Information
The modification event is designated as a cisgenic event because the gene is derived from the same organism (H. vulgare).