Cisgenic barley with improved phytase activity | BCH-LMO-SCBD-114555 | Living Modified Organism | Biosafety Clearing-House

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last updated: 21 Mar 2019
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Cisgenic barley with improved phytase activity
EN
PaPhy07
No
The modified barley has increased phytase activity due to the presence of an additional copy of the phytase gene (cisgenic). The increase phytase activity increases the bioavailability of phosphorous within the mature barley grains.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cv. Golden Promise
EN
  • Barley modified to produce phytase
    | Institute of Experimental Botany AS CR(IEB) | Resistance to herbicides (Glufosinate)
Characteristics of the modification process
pTiT37
EN
  • Agrobacterium-mediated DNA transfer
 
2.180 kb
 
 
2.270 kb
 
 
0.760 kb
 
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-114546-2 Barley Phytase | Hordeum vulgare (Barley, HORVU)
    Protein coding sequence | Changes in quality and/or metabolite content (Protein and amino acids, Vitamins)
  • BCH-GENE-SCBD-114547-2 Barley Phytase Promoter | Hordeum vulgare (Barley, HORVU)
    Promoter
  • BCH-GENE-SCBD-114549-2 Barley Phytase Terminator | Hordeum vulgare (Barley, HORVU)
    Terminator
The barley (Hordeum vulgare) Phytase gene (HvPAPhy_a) is under the control of the native promoter. Transcription terminates at the native terminator. The additional copy of the phytase gene leads to an increase in the phytase activity in the mature grains.

Note:
- No selection genes or vector-backbone sequences were present in the modified barley line.
- The modified barley line had not previously been modified.
EN
LMO characteristics
  • BCH-GENE-SCBD-114546-2 Barley Phytase | Hordeum vulgare (Barley, HORVU)
    Protein coding sequence | Changes in quality and/or metabolite content (Protein and amino acids, Vitamins)
An additional copy of HvPAPhy_a leads to an increased expression and activity of HvPAPHY_A in the mature grains.
EN
  • Feed
Detection method(s)
EN
Additional Information
The modification event is designated as a cisgenic event because the gene is derived from the same organism (H. vulgare).
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1