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Gene and DNA Sequence
Record information and status
Record ID
114683
Status
Published
Date of creation
2019-05-10 17:01 UTC (austein.mcloughlin@cbd.int)
Date of last update
2019-06-19 14:42 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-06-19 14:42 UTC (austein.mcloughlin@cbd.int)

General information
Name of the gene or DNA sequence
Recombinant glycolate dehydrogenase (fused subunits DEF)
Alternate Genetic Element Name (synonyms)
DEFp
Abbreviation
GlyDH
Category of DNA sequence
  • Protein coding sequence
Is the gene / DNA sequence is a synthetic molecule?
No
Donor organism
Donor organism
Escherichia coli - ECOLX
Characteristics of the protein coding sequence
Biological function
Glycolate dehydrogenase converts 2-phosphoglycolate to glycerate and CO2. In plant biotechnology applications, the enzyme can be used used to increase photosynthetic rates by reducing the the toxic effects of photorespiration (Ribulose-1,5-bisphosphate-caboxylase/-oxygenase (Rubisco) to binding O2 instead of CO2).

The recombinant glycolate dehydrogenase polyprotein (DEFp) by fusing the corresponding E. coli glcD (1493bps),glcE (1046 bps), and glcF (1220 bps) cDNAs with intervening flexible (Gly4Ser) 3 linkers. The fused polyprotein ensures the protein subunits are produced in stoichiometric amounts.
Related trait(s) or use(s) in biotechnology
  • Changes to photosynthesis and photorespiration

Records referencing this document (2)
IDDescription
2record(s) found
Modified Organism2 records