Hybrid aspen with modified autumn phenology | BCH-LMO-SCBD-114965 | Living Modified Organism | Biosafety Clearing-House
CBD / BCH / Search / Record

Loading...
Living Modified Organism (LMO)
  |  
BCH-LMO-SCBD-114965-2   |   PDF   |   Print   |  
| 
|  Close
Decisions on the LMO Risk Assessments  
last updated: 19 Jun 2019
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Hybrid aspen with modified autumn phenology
EN
oatPHYAox
No
The modified hybrid aspen overexpresses an oat (Avena sativa) PHYTOCHROMEA (AsPHYA) complementary DNA (cDNA) gene sequence that confers altered autumn phenology. The  hybrid aspens show shorter internode length due to constitutive auxin and gibberellin synthesis, insensitivity to day length, a shorter period of leaf movements, and are not frost hardy (cold sensitive) if grown during cycles of light/dark. Furthermore, overexpression of PHYA prevented leaf abscission.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Populus tremula x Populus tremuloides wild-type clone T89
EN
Characteristics of the modification process
pPCV702
EN
  • Agrobacterium-mediated DNA transfer
 
0.920 kb
 
 
3.700 kb
 
 
0.270 kb
 
 
0.290 kb
 
 
0.798 kb
 
 
0.410 kb
 
 
0.105 kb
 
 
0.861 kb
 
 
0.243 kb
 
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The transformation of the aspen trees introduced three gene cassettes: an oat (Avena sativa) PHYTOCHROMEA (AsPHYA) overexpression cassette, an Escherichia coli neomycin phosphotransferase II (NptII) cassette, and an E. coli beta-lactamase (AmpR) cassette.

The overexpression of AsPHYA is driven by the Cauliflower Mosaic Virus 35S promoter and terminates at the Agrobacterium tumefaciens nopaline synthase (nos) gene terminator. The coding sequence of AsPHYA was created through the reverse transcription of oat mRNA. Therefore, introns are not present in the coding sequence.

Transcription of NptII is under the control of the nos promoter and the A. tumefaciens gene 4 terminator. NptII is used as a selectable marker for plant transformation events as it confers kanamycin resistance.

AmpR expression is under the control of the native beta-lactamase promoter and terminator. Expression is restrained to bacterial systems and transcription is not expected to occur in eukaryotic systems. This allows for ampicillin selection during bacterial transformation. The cassette was taken from the pBR322 plasmid.
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1

Quick links

About the BCH About the Cartagena Protocol Supplementary Protocol

Sign in Search records Submit records
Registries: LMO-Organism-Gene Country Profiles
National Report Analyzer

Forums BCH Announcements

Need help?

Knowledge Base FAQs Training Materials Training Site

Contact us at: bch@cbd.int
BCH Biosafety Clearing-House
Subscribe to e-mail notifications
Subscribe to our newsletter