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Modified Organism
Hybrid aspen with modified autumn phenology
Record information and status
Record ID
114965
Status
Published
Date of creation
2019-06-19 18:50 UTC (austein.mcloughlin@cbd.int)
Date of last update
2019-06-19 19:38 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-06-19 19:38 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Hybrid aspen with modified autumn phenology
Transformation event
oatPHYAox
Developer(s)
Dr Stefan Jansson
Professor in plant cell and mollecular biology
Umeå Plant Science Centre, Physioogical Botany
Swedish Agricultural University, Umeå (SLU)
Umeå University
Umeå Plant Science Centre
Physiological Botany
Umeå, Umeå
Sweden, 901 87
Phone:+4690-7865354
Email:stefan.jansson@umu.se
Description
The modified hybrid aspen overexpresses an oat (Avena sativa) PHYTOCHROMEA (AsPHYA) complementary DNA (cDNA) gene sequence that confers altered autumn phenology. The  hybrid aspens show shorter internode length due to constitutive auxin and gibberellin synthesis, insensitivity to day length, a shorter period of leaf movements, and are not frost hardy (cold sensitive) if grown during cycles of light/dark. Furthermore, overexpression of PHYA prevented leaf abscission.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Populus tremula x Populus tremuloides - Hybrid aspen
Point of collection or acquisition of the recipient organism
Populus tremula x Populus tremuloides wild-type clone T89
Characteristics of the transformation process
Vector
pPCV702
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.92 Kb
 
 
Phytochrome A
3.70 Kb
 
 
Nopaline Synthase Gene Terminator
0.27 Kb
 
 
Nopaline Synthase Gene Promoter
0.29 Kb
 
 
Neomycin Phosphotransferase II
0.80 Kb
 
 
Gene 4 transcription terminator
0.41 Kb
 
 
Beta-lactamase promoter
0.11 Kb
 
 
Beta-lactamase gene
0.86 Kb
 
 
Beta-lactamase terminator
0.24 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The transformation of the aspen trees introduced three gene cassettes: an oat (Avena sativa) PHYTOCHROMEA (AsPHYA) overexpression cassette, an Escherichia coli neomycin phosphotransferase II (NptII) cassette, and an E. coli beta-lactamase (AmpR) cassette.

The overexpression of AsPHYA is driven by the Cauliflower Mosaic Virus 35S promoter and terminates at the Agrobacterium tumefaciens nopaline synthase (nos) gene terminator. The coding sequence of AsPHYA was created through the reverse transcription of oat mRNA. Therefore, introns are not present in the coding sequence.

Transcription of NptII is under the control of the nos promoter and the A. tumefaciens gene 4 terminator. NptII is used as a selectable marker for plant transformation events as it confers kanamycin resistance.

AmpR expression is under the control of the native beta-lactamase promoter and terminator. Expression is restrained to bacterial systems and transcription is not expected to occur in eukaryotic systems. This allows for ampicillin selection during bacterial transformation. The cassette was taken from the pBR322 plasmid.
LMO characteristics
Modified traits
  • Inhibited leaf abscission
  • Sensitivity to cold
Common use(s)
  • Research