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Living Modified Organism
(LMO)
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Herbicide-tolerant sugar cane
EN
TUC-873RH-7
Yes
TUC-873RH-7
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- Organization: Estación Experimental Agroindustrial Obispo Colombres (EEAOC) | BCH-CON-SCBD-115056-1San Miguel de Tucumán, TucumánOrganization:Estación Experimental Agroindustrial Obispo Colombres (EEAOC)
T4101XAC, ArgentinaPhone: +54 381 452 1000,Fax: +54 381 452 1008,Email:Website: http://www.eeaoc.org.ar,
Sugarcane (Saccharum sp.) was modified to contain Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase for tolerance to glyphosate. An Escherichia coli neomycin phosphotransferase II cassette was also included for kanamycin selection during transformation.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-100332-4 Organism Saccharum sp. (Sugar cane)Crops
Sugarcane cultivar RA 87-3
EN
pBluescript II KS(+)
EN
- Biolistic / Particle gun
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)Promoter
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BCH-GENE-SCBD-103899-3 Chloroplast Transit Peptide 4 | Petunia hybrida (Petunia, PETHY)Transit signal
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
The modified sugarcane contains two gene cassettes: Escherichia coli (nptII) and Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate (cp4-epsps).
The nptII coding sequence is under control of a Zea mays ubiquitin promoter and an A. tumefaciens nopaline synthase terminator. Due to the constitutive nature of the promoter, transcription is expected to occur at elevated levels in all tissue types.
The cp4-epsps coding sequence is under control of an Oryza sativa actin 1 promoter and Cauliflower mosaic virus 35S terminator. To the 5' end of the cp4-epsps sequence is a Petunia hybrida chloroplast transit peptide 4 sequence to target the translated EPSPS protein to the chloroplast. The rice promoter is expected to facilitate high levels of transcription in all tissue types.
Note:
- Southern blot analysis confirmed that 9 insertions of the transgenes occurred.
- Whole genome sequencing, Sanger sequencing, and PCR analysis confirmed that the vector backbone was not present in the genome, and that no deletions nor rearrangements occurred.
EN
The nptII coding sequence is under control of a Zea mays ubiquitin promoter and an A. tumefaciens nopaline synthase terminator. Due to the constitutive nature of the promoter, transcription is expected to occur at elevated levels in all tissue types.
The cp4-epsps coding sequence is under control of an Oryza sativa actin 1 promoter and Cauliflower mosaic virus 35S terminator. To the 5' end of the cp4-epsps sequence is a Petunia hybrida chloroplast transit peptide 4 sequence to target the translated EPSPS protein to the chloroplast. The rice promoter is expected to facilitate high levels of transcription in all tissue types.
Note:
- Southern blot analysis confirmed that 9 insertions of the transgenes occurred.
- Whole genome sequencing, Sanger sequencing, and PCR analysis confirmed that the vector backbone was not present in the genome, and that no deletions nor rearrangements occurred.
EN
- Food
- Biofuel
EN
EN
- [Spanish] DOC DEC TUC-873RH-7 V1 versión final firmado 17-09-15.pdf [ English ]
- EUginius - TUC-873RH-7 [ English ]
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