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Modified Organism
DBN-Ø9ØØ4-6 - Glyphosate and Glufosinate tolerant soybean
Record information and status
Record ID
115067
Status
Published
Date of creation
2019-07-26 14:37 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-07-26 14:38 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Glyphosate and Glufosinate tolerant soybean
Transformation event
DBN4003
Unique identifier
DBN-Ø9ØØ4-6
Developer(s)
Bioceres S.A.
Ocampo 210 bis
Predio CCT Rosario
(2000) Rosario, Santa Fe, Argentina
Tel: (54) 341 4861100
Rosario
Argentina
Phone:+ 54 341 4861100
Url:Bioceres (INDEAR)
Description
The soybean was modified to express Agrobacterium tumefaciens CP4 EPSPS and Streptomyces viridochromogenes PAT proteins for tolerance to glyphosate and glufosinate herbicides, respectively. The bacterial CP4 EPSPS functions similarly to the endogenous enzyme, but has a greatly reduced affinity for glyphosate. The PAT protein detoxifes L-phosphinothricin (glufosinate) via the acetylation of the primary amino group.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Characteristics of the transformation process
Vector
pDBN9003
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Elongation Factor 1 alpha promoter
1.01 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
rbcS-E9 gene terminator
0.64 Kb
 
 
CaMV 35S promoter
0.53 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
CP4 EPSPS:
Transcription of the Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (cp4 epsps) commences from the Glycine max Elongation factor 1 alpha promoter and terminates at the Pisum sativum  ribulose-1,5-bisphosphate carboxylase, small subunit (rbcS E9) terminator. An Arabidopsis thaliana chloroplast peptide 2 segment was added to the 5' end of cp4 epsps to target the final protein product to the chloroplast. The soy EF1α promoter is expected to promote high levels of transcription.

PAT:
Streptomyces viridochromogenes Phosphinothricin N-acetyltransferase (pat) gene is under control of the Cauliflower Mosaic Virus (CaMV) 35S promoter and terminator. Elevated levels of transcription of pat are expected due to the CaMV 35S promoter. The protein is expected to remain in the cytoplasm of the plant cells.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel
Additional Information