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Modified Organism
SPS-ØØV11-6 - Innate® Invigorate Snowden
Record information and status
Record ID
115075
Status
Published
Date of creation
2019-07-30 20:11 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-07-30 20:11 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Innate® Invigorate Snowden
Transformation event
V11
Unique identifier
SPS-ØØV11-6
Developer(s)
J.R. Simplot Company
5369 West Irving Street 
Boise, ID
United States of America, 83706
Phone:+1 (208) 780-6066
Description
The potato variety Snowden was modified for reduced blackening of the tuber and decreased levels of reducing sugars through the downregulation of the endogenous asparagine synthase 1 (Asn1), water dikinase R1 (R1), starch phosphorylase L (PhL), and polyphenol oxidase 5 (PPO5) by RNA interference. Silencing of PPO5 reduces the browning and blackening of the tuber flesh. Down-regulation of R1, Asn1, and PhL decrease the concentration of reducing sugars and thus decrease the potential of acrylamide formation via the Maillard reaction.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Solanum tuberosum var. Snowden
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SPS-ØØE24-2 - Innate™ Russet Burbank Potato
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SPS-ØØJ55-2 - Innate™ Atlantic Potato
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Characteristics of the transformation process
Vector
pSIM1278
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
ADP glucose pyrophosphorylase gene promoter
2.26 Kb
 
 
Asparagine synthetase-1 gene
0.40 Kb
 
 
Polyphenol oxidase 5 gene
0.14 Kb
 
 
Spacer sequence
0.16 Kb
 
 
Polyphenol oxidase 5 gene
0.14 Kb
 
 
Asparagine synthetase-1 gene
0.40 Kb
 
 
Granule bound starch synthase gene promoter
0.69 Kb
 
 
ADP glucose pyrophosphorylase gene promoter
2.26 Kb
 
 
Phosphorylase-L gene promoter
0.51 Kb
 
 
Alpha-glucan water dikinase R1 gene promoter
0.53 Kb
 
 
Spacer sequence
0.26 Kb
 
 
Alpha-glucan water dikinase R1 gene promoter
0.53 Kb
 
 
Phosphorylase-L gene promoter
0.51 Kb
 
 
Granule bound starch synthase gene promoter
0.95 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The modified potato contains two RNA interference (RNAi) cassettes for the potato genes: asparagine synthase-1 (Asn1) - polyphenol oxidase 5 (PPO5) and phosphorylase-L (PhL) - Alpha-glucan water dikinase R1 (R1).

Asn1-PPO5:
Transcription commences from both promoters, the potato ADP glucose pyrophosphorylase promoter (P-Agp) and the potato granule bound starch synthase promoter (P-Gbss), which are in a convergent orientation relative to each other. The transcripts produced have inverted repeats of Asn1 and PPO5 segments, separated by a spacer sequence derived from the potato genome. After transcription, the transcripts form double stranded RNA (dsRNA) molecules due to the homology of the sequences. The RNA molecules are then sufficient to trigger an RNAi response.

pPhL-R1:
Transcription is also directed from the convergent promoters P-Agp and P-Gbss. The RNA molecules contain inverted repeats of the promoter segments of PhL and R1, as well as a spacer derived from the potato genome. Post-transcription, the RNA molecules can form double stranded structures due to the sequence homology within the transcripts and trigger an RNAi response.

Note:
- The promoters are tuber-specific and expected to be most active in the tuber tissue.
- The transcripts are not expected to be translated into proteins.
- No specific terminators were added to the cassettes and thus the transcripts are not expected to have poly(A) tails.
- No marker genes (e.g. kanamycin or hygromycin resistance) are present.
- Southern blotting confirmed that the V11 genome contains a single T-DNA insertion.
- No vector backbone was incorporated into the V11 genome.
LMO characteristics
Modified traits
How the expression of the gene(s) was affected
Due to the formation of the dsRNA structures, RNA interference is triggered. The dsRNA is processed into small interfering RNA (siRNA), which can direct degradation of transcripts that share homology to the siRNA. The degradation of the endogenous mRNA results in the silencing of gene expression of the following endogenous genes: Asn1, PPO5, PhL, and R1.
Common use(s)
  • Food