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Information Resource
Record information and status
Record ID
115098
Status
Published
Date of creation
2019-08-14 19:06 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-08-14 19:06 UTC (austein.mcloughlin@cbd.int)

General Information
Title
MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market
Author
Marie-Alice Fraiture, Gabriella Ujhelyi, Jaroslava Ovesná, Dirk Van Geel, Sigrid De Keersmaecker, Assia Saltykova, Nina Papazova, Nancy H. C. Roosens
Author’s contact information
Correspondence and requests for materials should be addressed to Nancy H. C. Roosens (email: nancy.roosens@sciensano.be)
Language(s)
  • English
Publication date
2019-05-09
Subject
Summary, abstract or table of contents
In order to characterize unauthorized genetically modified petunia, an integrated strategy has been applied here on several suspected petunia samples from the European market. More precisely, DNA fragments of interest were produced by DNA walking anchored on key targets, earlier detected by realtime PCR screening analysis, to be subsequently sequenced using the MinION platform from Oxford Nanopore Technologies. This way, the presence of genetically modified petunia was demonstrated via the characterization of their transgene flanking regions as well as unnatural associations of elements from their transgenic cassette.
Thematic areas
Information on Organisms or LMOs
Organism(s) identification
Petunia hybrida - Petunia, PETHY
Gene(s) identification
CaMV 35S promoter - Cauliflower mosaic virus - CaMV
Nopaline Synthase Gene Promoter - Agrobacterium tumefaciens - Agrobacterium
CaMV 35S terminator - Cauliflower mosaic virus - CaMV
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
Identifier
DOI: 10.1038/s41598-019-43463-5
Publisher and its location
Scientific Reports
Format
PDF (12 pages)
Source
Article from journal
Keywords and any other relevant information
Unauthorized, LMO, petunia, PCR, sequencing