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Modified Organism
Maize with altered growth and tolerance to herbicides
Record information and status
Record ID
115131
Status
Published
Date of creation
2019-08-20 19:57 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-08-20 19:57 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Maize with altered growth and tolerance to herbicides
Transformation event
pUBIL-GA20OX1 × pGA2ox-PLA1
Developer(s)
Dr. Hilde Nelissen
Departement Plantensysteembiologie
VIB - Universiteit Gent (VIB-UGent)
Technologiepark 927
Gent
Belgium, B-9052
Phone:+32 9 2446611
Email:vib@vib.be
Url:VIB
Description
The modified maize plant was produced through crossing of two modified lines (stacked event) that demonstrate modified growth characteristics resulting from the additional expression of two genes: (1) the Arabidopsis thaliana GA20oxidase (gibberellin 2-beta-dioxygenase) under the control of a Zea mays (maize) pUBIL promoter and (2) the maize PLA1 gene (plastochron1; also known as the 'KLUH' gene, or CYP78A1 gene) under the control of the maize GA2oxidase promoter.

The GA20oxidase gene is involved in the production of bio-active gibberellins, which cause a cellular increase in cellulose, lignin, and cell wall residue. The PLA1 gene codes for a cytochrome P450 mono-oxygenase enzyme that is involved in the production of factors that control cell proliferation. As a result of the modification, the plants have more rapid and prolonged growth, which leads to plants that have significantly larger leaves, a higher biomass, and a significantly higher cob yield. The modification also results in more branches being formed on the tassel and a shorter anthesis-silking interval.

The plant also contains the bar gene which produces the Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (PAT) protein as a selection marker gene to confer tolerance to the herbicide glufosinate.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Maize with altered growth and tolerance to herbicides
Prof. Dr. Frank Van Breusegem Changes in physiology and/or production - Growth rate Resistance to herbicides - Glufosinate
Maize with altered growth and tolerance to herbicides
Dr. Hilde Nelissen Changes in physiology and/or production - Yield, Growth rate, Reproduction Resistance to herbicides - Glufosinate Tolerance to abiotic stress - Cold / Heat, Drought
Point of collection or acquisition of the recipient organism
Cultivar/ Breeding line: inbred line B104
Characteristics of the transformation process
Vector
pMBb7Fm21GW-UBIL; pBbm42GW7
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV 35S terminator
0.23 Kb
 
 
Gibberellin 2-beta-dioxygenase gene
1.13 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Nopaline Synthase Gene Terminator
0.27 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Omega 5' untranslated leader
0.09 Kb
 
 
CaMV 35S promoter
0.94 Kb
 
 
GA2-oxidase promoter
2.05 Kb
 
 
Plastochron 1
1.83 Kb
 
 
CaMV 35S terminator
0.27 Kb
 
 
Nopaline Synthase Gene Terminator
0.27 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Omega 5' untranslated leader
0.09 Kb
 
 
CaMV 35S promoter
0.94 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Genetic elements associated with pMBb7Fm21GW-UBIL:
Transcription of the Arabidopsis thaliana gibberellin 2-beta-dioxygenase (GA20 oxidase) is under control of the Zea mays (maize) ubiquitin gene promoter and the Cauliflower Mosaic Virus (CaMV) 35S terminator.

Transcription of the herbicide tolerance gene phosphinothricin N-acetyltransferase (bar; pat) from Streptomyces hygroscopus commences from the CaMV 35S promoter and ends at the Agrobacterium tumefaciens nopaline synthase terminator. The transcript contains a Tobacco Mosaic Virus 5' untranslated omega leader sequence that promotes the translation of PAT.

Genetic elements associated with pBbm42GW7:
Transcription of the maize plastochron1 (ZmPla1) gene commences from the maize GA2-oxidase (GA2ox) promoter and terminates at the Cauliflower Mosaic Virus (CaMV) 35S terminator. The transcribed sequence of ZmPla1 was derived from the reverse transcription of the messenger RNA for ZmPLA1 (cDNA). Thus, no introns are present in the coding sequence that was integrated into the genome.

In the other gene cassette, transcription of bar starts from the CaMV 35S promoter and ends at the A. tumefaciens nopaline synthase terminator. The transcript contains a 5' untranslated Tobacco Mosaic Virus omega leader sequence to enhance translation of the bar gene.

Kindly refer to the parental records for more information.
LMO characteristics
Modified traits
Other gene(s) whose expression was affected by the transformation
Plastochron 1 - Zea mays - Maize, Corn, MAIZE
Changes in physiology and/or production - Yield, Growth rate, Reproduction
Tolerance to abiotic stress - Cold / Heat, Drought
How the expression of the gene(s) was affected
The expression of the ZmPla1 occurs from two sources: the native, endogenous gene and the integrated gene cassette. Thus, it is expected that an elevated level of transcript and protein would result compared to a non-modified maize.
Common use(s)
  • Food
  • Feed
  • Research
  • Biofuel