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Modified Organism
Cassava brown streak disease-resistant cassava
Record information and status
Record ID
115143
Status
Published
Date of creation
2019-08-23 14:08 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-08-23 14:08 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Cassava brown streak disease-resistant cassava
Transformation event
D5001-985013
Developer(s)
National Root Crops Research Institute (NRCRI)
Km 8 Ikot Ekpene Road, PMB 7006
Umuahia, Abia
Nigeria
Phone:+234 (0)8168983790,+2349035068714
Email:info@nrcri.gov.ng,Ihuomaumezurumba@yahoo.com
Url:National Root Crops Research Institute
Description
Cassava (Manihot esculenta Crantz) was modified for RNA interference-mediated resistance to Cassava Brown Streak Disease. The modified cassava expresses a hairpin RNA (hpRNA) cassette that contains portions of the coat proteins of Cassava Brown Streak Virus and Ugandan Cassava Brown Streak Virus, the causal agents of Cassava Brown Streak Disease. Transcription of the hpRNA form this cassette guides host cell machinery for targeted degradation of infecting viral transcripts.

A selectable marker, Escherichia coli neomycin phosphotransferase II, was also included for selection of transformants using kanamycin.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Manihot esculenta - Cassava, Brazilian arrowroot, Yuca, Manioc, Mandioca, MANES
Point of collection or acquisition of the recipient organism
Cassava cultivar: TMS 98/0505
Related LMOs
Viral resistant cassava with increased levels of zinc and iron
National Root Crops Research Institute Changes in quality and/or metabolite content Increased levels of iron Resistance to antibiotics - Kanamycin Tolerance to abiotic stress Tolerance to excess iron
Show detection method(s)
Viral resistant cassava with increased levels of zinc and iron
Changes in quality and/or metabolite content Increased levels of iron Resistance to antibiotics - Kanamycin Tolerance to abiotic stress Tolerance to excess iron
Characteristics of the transformation process
Vector
p5001
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Nopaline Synthase Gene Terminator
0.27 Kb
 
 
Coat protein
0.90 Kb
 
 
Coat Protein
0.90 Kb
 
 
Pyruvate orthophosphate dikinase, Intron 3
0.78 Kb
 
 
Coat Protein
0.90 Kb
 
 
Coat protein
0.90 Kb
 
 
CsVMV promoter
0.52 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Neomycin Phosphotransferase II
0.80 Kb
 
 
CaMV Enhanced 35S promoter
0.39 Kb
 
 
CaMV Enhanced 35S promoter
0.39 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
RNA interference cassette:
Transcription of the cassette produces a hairpin RNA (hpRNA). RNA polymerase is recruited to the Cassava vein mosaic virus promoter and then transcribes: two segments of Cassava Brown Streak Virus (CBSV) and the Ugandan Cassava Brown Streak Virus (UCBSV) coat protein (sense orientation), the Flaveria trinervia pyruvate orthophosphate dikinase intron 3 (PDK), and CBSV and UCBSV coat protein (anti-sense orientation). Transcription stops at the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The produced RNA then can form a double stranded segment due to the interstrand homology between the CBSV and UCBSV coat protein segments in inverted orientations and the flexible linker/loop, PDK. The double stranded segment of the hpRNA is sufficient to elicit an RNA interference (RNAi) response. The host cell machinery then processes the hpRNA into small interfering RNA, which is used to guide the targeted degradation of RNA molecules with sequence homology. In this case, infecting viral transcripts are degraded by the host cell.

Please note all genetic elements were in the anti-sense orientation.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
  • Resistance to CBSV
  • Resistance to UCBSV
Common use(s)
  • Food
  • Research
Detection method(s)
Additional information
Due to the processing of the hpRNA by the host cell DICER protein, it is expected that small interfering RNA 21 to 24 base pairs in length will be present instead. Additionally, the cellular processing means that no proteins are expected to be produced from the hpRNA.
Additional Information
Additional Information
Please note that several lines have been created using the transformation vector p5001. The following transformation events are expected to contain similar genetics:

D5001-985013, D5001-985019, D5001-985022, D5001-985024, D5001-985025, D5001-985026, D5001-985028, D5001-985029, D5001-985030, D5001-985031, D5001-985033 and D5001-985034.

The transformation event is thus a placeholder until further information is uncover and/or a line has been selected for commercialization.

Records referencing this document (2)
IDDescription
2record(s) found
Modified Organism2 records