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Modified Organism
Gypsophila modified for altered flower colour
Record information and status
Record ID
115181
Status
Published
Date of creation
2019-09-26 15:39 UTC (austein.mcloughlin@cbd.int)
Date of last update
2019-09-26 15:45 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-09-26 15:45 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Gypsophila modified for altered flower colour
Transformation event
PAP1
Developer(s)
Mr Gavriel Danziger
Imaginature Ltd
Israel
Israel
Phone:+97239602525
Email:info@imaginature.co.il
Description
Baby's Breath (Gypsophila paniculata; cut gypsophila) was modified to express Arabidopsis thaliana Production of Anthocyanin Pigment 1, conferring coloured flowers due to the accumulation of anthocyanin pigment in the vacuoles of the cells. The colour of the flowers was also noted to remain stable. The modified flower also contains an Escherichia coli neomycin phosphotransferase II cassette as a selectable marker used during transformation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gypsophila paniculata - Baby's Breath, Common gypsophila
Characteristics of the transformation process
Vector
pCGN1559
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.39 Kb
 
 
PRODUCTION OF ANTHOCYANIN PIGMENT 1
1.55 Kb
 
 
Octopine Synthase Gene Terminator
0.91 Kb
 
 
CaMV 35S promoter
0.40 Kb
 
 
Neomycin Phosphotransferase II
0.76 Kb
 
 
Tumour Morphology Large gene terminator
1.42 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Pigment production
Transcription of the Arabidopsis thaliana PRODUCTION OF ANTHOCYANIN PIGMENT 1(AtPAP1) occurs from the Cauliflower Mosaic Virus (CaMV) 35S promoter and terminates at the Agrobacterium tumefaciens optopine synthase gene terminator. High levels of AtPaP1 expression are expected due to the constitutive activity of the CaMV 35S promoter.

Selectable marker
Transcription of the Escherichia coli neomycin phosphotransferase II (nptII) starts from another CaMV 35S promoter and stops at the A. tumefaciens tumour morpholoigy large gene terminator. Similar to the AtPAP1 gene, high levels of transcription are expected to be achieved via the CaMV 35S promoiter.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Ornamental
Detection method(s)
Additional information
AtPAP1
High levels of the AtPAP1 transcript and AtPAP1 protein are expected.

nptII
High levels of NPTII transcript and NPTII protein are expected.
Additional Information
Other relevant website address or attached documents