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Information Resource
Record information and status
Record ID
115232
Status
Published
Date of creation
2019-10-22 19:26 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-10-22 19:26 UTC (austein.mcloughlin@cbd.int)

General Information
Title
Detection of transgenic rice line TT51-1 in processed foods using conventional PCR, real-time PCR, and droplet digital PCR
Author
Xiaofu Wang, Ting Tang, Qingmei Miao, Shilong Xie, Xiaoyun Chen, Jun Tang, Cheng Peng, Xiaoli Xu, Wei Wei, Zhaotong You, Junfeng Xu
Language(s)
  • English
Publication date
2018-11-28
Subject
Summary, abstract or table of contents
To assess the effects of food processing on the detection and quantification of transgenic rice TT51-1 in processed food by polymerase chain reaction (PCR) technology, we monitored the presence of TT51-1 components in rice crackers at different processing stages using conventional PCR, quantitative real-time PCR (qPCR), and droplet digital PCR (ddPCR) with standard or validated primers and probes. In conventional PCR, relatively longer amplification targets, such as the Bacillus thuringiensis (Bt) gene (301 bp) and the event-specific target (274 bp), were barely detected in baked, fried or microwaved samples. In qPCR, the amplification fluorescence signal was detected in boiled, dried, baked, and microwaved samples, but barely observed in fried samples. Conventional PCR with the same primers used in qPCR detected the corresponding shorter targets in all samples. The conventional PCR results were mainly consistent with the results of qPCR. The results indicate that food processing directly affects the detection of transgenic components, and suggest that relatively shorter fragments should be selected as the amplification targets for this type of analysis. We established qPCR and duplex ddPCR methods for quantifying TT51-1. The results of an orthogonal experiment indicated that the optimal conditions for TT51-1/PLD duplex ddPCR were 500/250 nM of primers/probe combined with 58 °C annealing temperature. Both methods were feasible for quantitative detection of TT51-1 in processed samples, with duplex ddPCR being a more attractive method for detecting transgenic components in processed food due to its stability, accuracy, PCR inhibitor resistance, and the lack of a need for reference materials.
Thematic areas
Information on Organisms or LMOs
LMO(s) identification
HZU-HHØØ1-9 - Insect resistent rice
Resistance to antibiotics - Ampicillin, Hygromycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) - Cotton bollworm (Helicoverpa spp.) Selectable marker genes and reporter genes
Show detection method(s)
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
Identifier
DOI: 10.1016/j.foodcont.2018.11.032
Publisher and its location
Food Control
Format
PDF - 9 pages (2350 kB)
Source
Article from journal
Keywords and any other relevant information
Rice, food, PCR, detection