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Modified Organism
MON-89Ø34-3 × MON-8746Ø-4 - Drought-tolerant, insect-resistant maize
Record information and status
Record ID
115277
Status
Published
Date of creation
2019-11-22 16:25 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-11-22 16:25 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Drought-tolerant, insect-resistant maize
Transformation event
MON 87460 × MON 89034
Unique identifier
MON-89Ø34-3 × MON-8746Ø-4
Developer(s)
Bayer Crop Science Company
Bayer Crop Science Company
P.O. Box 79345-00200  Kenya
Nairobi
Kenya
Phone:+25478 6666087
Email:simonevans.njeru@bayer.com
Description
The drought-tolerant, insect-resistant maize (MON 87460 × MON 89034) was obtained through crossing the two maize event products: MON 87460 and MON 89034. The modified maize expresses Bacillus subtilis cold shock protein (from MON97460), which confers cold and drought tolerance, as well as the Bacillus thuringiensis insecticidal proteins Cry1A.105 and Cry2Ab2 (from MON 89034), which confer resistance to Lepidoptera pests. Additionally, a selectable marker for kanamycin resistance (neomycin phosphotransferase II) is expected to be present as it was used for selection of transformants during the generation of the parental MON 87460 line.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
MON-8746Ø-4 - Droughtgard™ Maize
Resistance to antibiotics - Kanamycin Tolerance to abiotic stress - Cold / Heat, Drought
Show detection method(s)
MON-89Ø34-3 - YieldGard™ VT Pro™
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
Characteristics of the transformation process
Vector
PV-ZMAP595; PV-ZMIR245
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Ti plasmid right border repeat
0.36 Kb
 
 
Rice actin 1 gene promoter
0.92 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Cold shock protein gene
0.20 Kb
 
 
Transcript 7 gene 3' untranslated region
0.51 Kb
 
 
loxP recombination site
0.03 Kb
 
 
CaMV 35S promoter
0.29 Kb
 
 
Neomycin Phosphotransferase II
0.79 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
loxP recombination site
0.03 Kb
 
 
Ti plasmid left border repeat
0.44 Kb
 
 
Ti plasmid right border repeat
0.36 Kb
 
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.06 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Cry1A.105
3.53 Kb
 
 
Terminator of the wheat heat shock protein 17.3
0.21 Kb
 
 
FMV 35S promoter
0.56 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Transit peptide and first intron of Rubisco SSU
0.40 Kb
 
 
Cry2Ab2
1.91 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ti plasmid left border repeat
0.44 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Genetic elements introduced from PV-ZMAP595
Two gene cassettes were integrated from this vector.
I. Transcription of Bacillus subtilis cold shock protein (CspB) begins from the Oryza sativa (rice) actin 1 promoter and ends at the Agrobacterium tumefaciens 3' untranslated region of transcript 7. Transcript contains the rice actin 1 intron at the 5' end. The intron is expected to enhance gene expression of CspB.

II. Transcription of the Escherichia coli neomycin phosphotransferase II (nptII) is under the control of the Cauliflower Mosaic Virus (CaMV) 35S promoter and the A. tumefaciens nopaline synthase terminator (nos). The gene cassette is flanked by Bacteriophage P1 locus of cross-over P1 (loxP) sites.

Please note
- The parental line contains a single insertion of the T-DNA from this vector.
- No vector backbone sequence was detected.
- The parental line contains intact genetic cassettes.

Genetic elements introduced from PV-ZMIR245
Two gene cassettes were present in the parental line.
III. Transcription of the Bacillus thuringiensis crystal 1A.105 (Cry1A.105) is commences from the CaMV enhanced 35S promoter and terminates at the Triticum aestivum (wheat) heat shock protein 17.3 terminator. The transcript contains a rice actin 1 intron and a wheat chlorophyll a/b-binding protein 5' leader  for enhanced gene expression.

IV. Transcription of B. thuringiensis Cry2Ab2 is under the control of the Figwort Mosaic Virus 35S promoter and the A. tumefaciens nos terminator. Zea mays heat shock protein 70 intron and transit peptide form Rubisco small subunit are also present in the transcript at the 5' end for enhanced gene expression and chloroplast targeting, respectively.

Please note
- The Cry2Ab2 coding sequence was optimized for expression in plants.
- An additional nptII cassette in reverse orientation was present in the pV-ZMIR245 vector and inserted as a secondary, unlinked T-DNA. During the development of the parental line, selective breeding was done to remove the nptII marker, resulting in a marker-free parental line.
- Southern blot analysis confirmed a single insertion and expression of the other T-DNA containing the genetic cassettes mentioned above (III and IV).
- DNA sequencing indicated that enhanced CaMV promoter did not contain the duplicated enhancer regions.
- No vector backbone was detected in the parental line.

For more information, kindly refer to the parental modified organism records
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Food
  • Feed