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Modified Organism
Papaya modified for resistance to Papaya Dieback Disease
Record information and status
Record ID
115340
Status
Published
Date of creation
2020-01-06 19:57 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-01-06 19:57 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Papaya modified for resistance to Papaya Dieback Disease
Transformation event
-
Developer(s)
Dr. Rogayah Sekeli
Plant Transformation and Molecular Biologist
Biotechnology and Nanotechnology Research Centre
Malaysian Agricultural Research and Development Institute (MARDI)
MARDI Headquarters, Persiaran MARDI-UPM
Serdang, Selangor
Malaysia, 43400
Phone:+603 89537313
Email:lynn@mardi.gov.my
Description
The Eksotika papaya (Carica papaya L. var. Eksotika) has been modified to have anti-pathogenic characteristics against Erwinia mallotivora, the causal agent of Papaya Dieback Diease, through the insertion of the two acyl-homoserine lactonase genes from Bacillus cereus CHB37 and Bacillus thuringiensis SP24. The two acyl-homoserine lactonase genes disrupt quorum sensing (bacterial cell-to-cell communication) through the hydrolysis of acyl-homoserine lactone and thus reducing expression of virulence genes and disease symptoms. Acyl-homoserine lactonase was previously found to specifically inhibit the growth of E. mallotivora in vitro, as well as in planta through the expression of B. cereus acyl-homoserine lactonase.

Additionally, Escherichia coli neomycin phophotransferase II, which confers resistance to kanamycin, was also inserted into the papaya genome to facilitate screening and identification of the transformants during the growth of the transgenic plants.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Carica papaya - Papaya, Pawpaw, Papaw, CARPA
Point of collection or acquisition of the recipient organism
Variety Eksotika
Characteristics of the transformation process
Vector
pCAMBIA2301
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.35 Kb
 
 
Acyl-homoserine lactonase CHB37
0.75 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S terminator
0.18 Kb
 
 
Neomycin Phosphotransferase II
0.80 Kb
 
 
CaMV Enhanced 35S promoter
0.68 Kb
 
 
CaMV 35S promoter
0.35 Kb
 
 
Acyl-homoserine lactonase SP24
0.75 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S terminator
0.18 Kb
 
 
Neomycin Phosphotransferase II
0.80 Kb
 
 
CaMV Enhanced 35S promoter
0.68 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Expression of acyl-homoserine lactonase genes:
Transcription of the Bacillus cereus CHB37 Acyl-homoserine lactonase is under the control of the Cauliflower Mosaic Virus 35S (CaMV 35S) promoter and the Agrobacterium tumefaciens nopaline synthase terminator (T-nos). Similarly, transcription of the Bacillus thuringiensis SP24 Acyl-homoserine lactonase is also under the control of the CaMV 35S promoter and the T-nos. High levels of expression are expected from the constitutive CaMV 35S promoter.

Selectable marker:
The vector contains an Escherichia coli neomycin phosphotransferase II cassette under transcriptional control of a CaMV 35S enhanced promoter and a CaMV 35S terminator. The CaMV 35S enhanced promoter is expected to promote constitutive expression of the neomycin phosphotransferase II transcript.

Please note:
The introduction of the two acyl-homoserine lactonase genes were a result of two separate Agrobacterium-mediated transformation events with the same vector.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
  • Resistance to Gram-negative bacteria
Common use(s)
  • Food

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record