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Information Resource
Record information and status
Record ID
115378
Status
Published
Date of creation
2020-02-06 15:03 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-02-06 15:04 UTC (austein.mcloughlin@cbd.int)

General Information
Title
Rapid detection of P-35S and T-nos in genetically modified organisms by recombinase polymerase amplification combined with a lateral flow strip
Author
Hua Liu, Jinbin Wang, Peng Li, Lan Bai, Junwei Jia, Aihu Pan, Xuanqi Long, Weidong Cui, Xueming Ting
Language(s)
  • English
Publication date
2019-07-17
Subject
Summary, abstract or table of contents
Abstract:
Recombinase polymerase amplification (RPA) was first reported in 2006 and has recently been shown to improve the detection of [living] modified organisms ([LMOs]). This study developed a more efficient RPA-based and lateral flow test strip (LFD)-based platform for the detection of P-35S and T-nos for [LMOs], circumventing the need for expensive instruments and technicians. Other methods were compared to our assay. The RPA forward and reverse primers were labeled with the fluorophore biotin, digoxin, and FITC at the 5′ end and quickly determined whether the sample contains P-35S and T-nos of [LMOs] using the RPA-LFD method at room temperature. Nine events of [LMOs] were collected to determine the specificity of the RPA-LFD method, including KMD1, MON531, Rf1, Kefeng6, RRS, Bt11, MON863, Bt176, and Ms1. The results showed that the limit of detection of RPA-LFD was 50 copies and 100 copies, which was consistent with the limit of detection of RPA-AGE. The specificity and stability among the nine events were consistent, respectively. Finally, the perfect fitness for the detection of the nine double-blind samples indicated that the detection method is practical. In conclusion, we developed a more sensitive, specific, and stable field screening method for determining the GMO content with large-scale field applications."
Thematic areas
Information on Organisms or LMOs
Gene(s) identification
CaMV 35S promoter - Cauliflower mosaic virus - CaMV
Nopaline Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium
LMO(s) identification
MON-ØØ531-6 - Bollgard™ cotton
Resistance to antibiotics - Kanamycin, Streptomycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
ACS-BNØØ1-4 - InVigor™ canola
Changes in physiology and/or production - Fertility restoration Resistance to antibiotics - Kanamycin Resistance to herbicides - Glufosinate
Show detection method(s)
MON-Ø4Ø32-6 - Roundup Ready™ soybean
Resistance to herbicides - Glyphosate
Show detection method(s)
SYN-BTØ11-1 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
MON-ØØ863-5 - YieldGard™ Rootworm™ maize
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Coleoptera (beetles)
Show detection method(s)
SYN-EV176-9 - NaturGard KnockOut™ maize
Resistance to antibiotics - Ampicillin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
ACS-BNØØ4-7 - InVigor™ canola
Changes in physiology and/or production - Reproduction - Male sterility Resistance to antibiotics - Kanamycin Resistance to herbicides - Glufosinate
Show detection method(s)
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
Identifier
DOI: 10.1016/j.foodcont.2019.106775
Publisher and its location
Food Control
Elsevier Ltd.
Format
PDF - 7 pages (1.5 MB)
Keywords and any other relevant information
recombinase polymerase amplification, lateral flow, isothermal, field application