Abstract
Oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) is amethod
that suppresses PCR amplification of target DNA in an ORN-specific
manner. In this study, we examined whether ORNi-PCR can be used to
enrich desirable DNA sequences from a DNA mixture by suppressing
undesirable DNA amplification. ORNi-PCR enriched edited DNA
sequences from a mixture of genomic DNA subjected to genome
editing. ORNi-PCR enabled more efficient analysis of the types of
insertion/deletion mutations introduced by genome editing. In
addition, ORNi-PCR reduced the detection of 16S ribosomal RNA (16S
rRNA) genes in 16S rRNA gene-based microbiome profiling, which
might permit a more detailed assessment of populations of other 16S
rRNA genes. Enrichment of desirable DNA sequences by ORNi-PCR may
be useful in molecular biology, medical diagnosis, and other
fields.
Methods manuscript
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