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Information Resource
Record information and status
Record ID
115391
Status
Published
Date of creation
2020-02-18 19:45 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-02-18 19:45 UTC (austein.mcloughlin@cbd.int)

General Information
Title
Development of a novel method for specific detection of genetically modified Atlantic salmon, AquAdvantage, using real-time polymerase chain reaction
Author
Keisuke Sogaa, Kosuke Nakamuraa, Takumi Ishigakia, Shinya Kimataa, Kiyomi Ohmorib, Masahiro Kishinec, Junichi Manoc, Reona Takabatakec, Kazumi Kittac, Hiroyuki Nagoyad, Kazunari Kondoa
Language(s)
  • English
Publication date
2019-08-26
Subject
Summary, abstract or table of contents
Abstract
Genetically modified ([LM]) Atlantic salmon, AquAdvantage (AquAd), was the first [LM] animal approved officially for human consumption. Many countries monitor the use of this product under their GM regulations, but a pragmatic system for AquAd-specific detection is needed. Here, we developed a real-time polymerase chain reaction method with high sensitivity for detection of AquAd in foods. This method showed high specificity for the AquAd transgene and the detection limit was 12.5-25 targeted DNA copies per test reaction. An interlaboratory study using the method developed demonstrated reproducibility at >0.1% (w/w) AquAd content.
Thematic areas
Information on Organisms or LMOs
LMO(s) identification
AquAdvantage® Salmon
Changes in physiology and/or production - Growth rate
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
Identifier
doi.org/10.1016/j.foodchem.2019.125426
Publisher and its location
Food Chemistry
Elsevier Ltd.
Format
PDF - 6 pages (833 KB)
Keywords and any other relevant information
fish; salmon; AquAdvantage; PCR; real-time PCR; detection