Record details

General Information

Title

A convenient renewable surface plasmon resonance chip for relative quantification of genetically modified soybean in food and feed

Author

Alexandra Plácido, Frederico Ferreira-da-Silva, José Roberto S. A. Leite, Noemí de-los-Santos-Álvarez, Cristina Delerue-Matos

Language(s)

English

Publication date

2020-02-26

Subject

Summary, abstract or table of contents

The cultivation of genetically modified organisms (GMO) continues to expand worldwide. Still, many consumers express concerns about the use of GMO in food or feed, and many countries have legislated on labelling systems to indicate the presence of GMO in commercial products. To deal with the increased number of GMO events and to address related regulations, alternative detection methods for GMO inspection are required. In this work, a genosensor based on Surface Plasmon Resonance under continuous flow was developed for the detection and quantification of a genetically modified soybean (event GTS 40-3-2). In a single chip, the simultaneous detection of the event-specific and the taxon-specific samples were achieved, whose detection limits were 20 pM and 16 pM, respectively. The reproducibility was 1.4%, which supports the use of the chip as a reliable and cost-effective alternative to other DNA-based techniques. The results indicate that the proposed method is a versatile tool for GMO quantification in food and feed samples.

Thematic areas

LMO use and transboundary movement
- LMOs for use as food or feed or for processing
- Handling, transport, packaging and identification
Scientific and technical issues
- Food and feed safety
- Detection

Information on Organisms or LMOs

Organism(s) identification

Record #10453

Glycine max - Soybean, Soya bean, Soya, SOYBN

Gene(s) identification

Record #14979

5-enolpyruvylshikimate-3-phosphate synthase gene - Agrobacterium tumefaciens - Agrobacterium

Resistance to herbicides - Glyphosate

LMO(s) identification

Record #14796

MON-Ø4Ø32-6 - Roundup Ready™ soybean

Resistance to herbicides - Glyphosate

Show detection method(s)

Additional Information

Type of resource

Article (journal / magazine / newspaper)

Identifier

https://doi.org/10.1371/journal.pone.0229659

Publisher and its location

PLOS ONE

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Rights

Open access

Format

PDF - 18 pages (2.4 MB)

Source

Peer-reviewed journal

Keywords and any other relevant information

Detection; quantification; soy; Surface Plasmon Resonance; renewable; food; feed

Access to the resource

Link to the resource(s)

A convenient renewable surface plasmon resonance chip for relative quantification of genetically modified soybean in food and feed.pdf

A convenient renewable surface plasmon resonance chip for relative quantification of genetically modified soybean in food and feed