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Modified Organism
MON-89Ø34-3 × MON-ØØ6Ø3-6 × DAS-4Ø278-9 - Herbicide-tolerant, Lepidoptera-resistant maize
Record information and status
Record ID
Date of creation
2020-04-15 13:45 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-04-15 13:45 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide-tolerant, Lepidoptera-resistant maize
Transformation event
MON89034 × NK603 × DAS40278
Unique identifier
MON-89Ø34-3 × MON-ØØ6Ø3-6 × DAS-4Ø278-9
The modified maize was obtained through traditional cross-breeding of parental lines modified for herbicide tolerance and insect resistance. The maize expresses the Bacillus thuringiensus proteins Cry1A.105 and Cry2Ab2 for resistance against Lepidoptera pests. The maize also expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase for tolerance to the herbicide glyphosate and Sphingobium herbicidovorans aryloxyalkanoate dioxygenase for tolerance to 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate (AOPP) acetyl coenzyme A carboxylase (ACCase) inhibitors.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
MON-89Ø34-3 - YieldGard™ VT Pro™
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
MON-ØØ6Ø3-6 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
DAS-4Ø278-9 - Enlist™ Maize
Dow AgroSciences GmbH Resistance to herbicides Tolerance to 2,4-Dichlorophenoxyacetic acid Tolerance to aryloxyphenoxypropionate
Show detection method(s)
Characteristics of the transformation process
PV-ZMIR245; PV-ZMGT32; pDAS1740
Techniques used for the modification
  • Cross breeding
Genetic elements construct
Ti plasmid left border repeat
0.24 Kb
CaMV Enhanced 35S promoter
0.30 Kb
5' untranslated leader from chlorophyll a/b-binding protein
0.06 Kb
Rice actin 1, intron
0.48 Kb
3.53 Kb
Heat shock protein 17.3 terminator
0.21 Kb
FMV 34S promoter
0.56 Kb
Hsp70 intron
0.80 Kb
Transit peptide and first intron of Rubisco SSU
0.40 Kb
1.91 Kb
Nopaline Synthase Gene Terminator
0.25 Kb
Ti plasmid right border repeat
0.23 Kb
Rice actin 1 gene promoter
0.80 Kb
Rice actin 1, intron
0.60 Kb
Chloroplast transit peptide 2
0.20 Kb
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
Nopaline Synthase Gene Terminator
0.30 Kb
CaMV Enhanced 35S promoter
0.60 Kb
Hsp70 intron
0.80 Kb
Chloroplast transit peptide 2
0.20 Kb
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
Nopaline Synthase Gene Terminator
0.30 Kb
RB7 matrix attachment region
1.17 Kb
Ubiquitin gene promoter
1.99 Kb
Aryloxyalkanoate dioxygenase gene
0.89 Kb
Per5 3' Untranslated Region
0.37 Kb
RB7 matrix attachment region
1.17 Kb
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from MON89034 vector PV-ZMIR245:
Maize line MON89034 expresses two Bt-toxins encoded by the Bacillus thuringiensis genes cry1A.105 and cry2Ab2.

Transcription of cry1A.105 begins at the Cauliflower Mosaic Virus (CaMV) Enhanced 35S promoter and finishes at the wheat (Triticum aestivum) wheat heat shock protein 17.3 terminator. The transcript initially includes (5' to 3'): wheat 5' untranslated leader from the chlorophyll a/b-binding protein, Oryza sativa (rice) actin 1 intron and cry1A.105. The wheat 5' untranslated leader sequence and the rice intron enhance the expression of cry1A.105.

Transcription of cry2Ab2 commences from the Figwort Mosaic Virus (FMV) 35S promoter and terminates at the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The transcript initially includes (5' to 3'): maize heat shock protein 70 (Hsp70) intron, maize transit peptide and first intron from the small subunit of Rubsico and cry2Ab32. The Hsp70 regulates and enhances gene expression, while the transit peptide targets cry2Ab2 to the chloroplast.

- The viral promoters are expected to be constitutively active and promote high levels of transcription.
- The coding sequence of cry2Ab2 was codon-optimized for expression within plant systems.
- A second T-DNA insertion (containing CaMV 35S promoter, Escherichia coli neomycin phosphotransferase and A. tumefaciens nos terminator) was initially inserted into the genome for kanamycin selection during transformation. However, once transformants were regenerated, the selectable marker was bred out of the parental line using convention breeding techniques.
- Southern blot analyses indicated a single copy of the cry1A.105 and the cry2Ab2 cassettes. No backbone plasmid DNA or nptII sequences were detected. PCR and DNA sequence analyses provided the complete DNA sequence of the insert and confirmed the organization of the elements within the insert. Furthermore, sequence analysis indicated that MON 89034 no longer has the duplicated enhancer elements compared to the original e35S promoter in PV-ZMIR245, possibly due to a recombination event that resulted in its deletion.

DNA insert from NK603 (MON-ØØ6Ø3-6), vector PV-ZMGT32:
The plant expression plasmid vector, PV-ZMGT32 contains two adjacent plant gene expression cassettes each containing a single copy of the Agrobacterium tumefaciens strain CP4 5-enolpyruvylshikimate-3-phosphate synthase (epsps). In the first expression cassette (5' end), the epsps gene is under transcriptional control of an Oryza sativa (rice) Actin 1 promoter and the A. tumefaciens nopaline synthase (nos) terminator. During transcription, a rice Actin 1 intron and an Arabidopsis thaliana chloroplast transit peptide 2 are included upstream (5') of the epsps coding sequence. The rice intron enhances EPSPS expression and the transit peptide targets EPSPS to the chloroplasts of the plant cells. The second epsps cassette is under control of the Cauliflower Mosaic Virus 35S enhanced promoter and the nos terminator. Similarly, transcription additionally includes a maize heat shock protein 70 intron and an A. thaliana chloroplast transit peptide 2. The heat shock protein intron also enhances expression of epsps.

The parental line (NK603) has one insertion site containing both epsps gene cassettes. No vector backbone (neomycin phosphotransferase and origin of replication) sequences were detected.

DNA insert from DAS40278 vector pDAS1740:
The LMO was generated using the Whiskers mediated transformation method. The aryloxyalkanoate dioxygenase-1 (aad-1)  is under the control of the Zea mays ubiquitin gene promoter and transcription terminates at Z. mays root preferential cationic peroxidase terminator. The aad-1 coding sequence was modified for plant-optimized expression.

- Southern blot analysis indicated that a single complete copy of the transformation cassette was stably integrated into the host genome at a single locus
- No integration of segments from the vector backbone occurred.

For more information, please refer to the parental records.
LMO characteristics
Modified traits
  • Tolerance to 2,4-Dichlorophenoxyacetic acid
  • Tolerance to aryloxyphenoxypropionate
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents