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Modified Organism
SYN-BTØ11-1 × DAS-Ø15Ø7-1 × SYN-Ø53Ø7-1 - Glufosinate tolerant, insect resistant maize
Record information and status
Record ID
115546
Status
Published
Date of creation
2020-04-22 19:09 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-04-22 19:09 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Glufosinate tolerant, insect resistant maize
Transformation event
Bt11 × TC1507 × 5307
Unique identifier
SYN-BTØ11-1 × DAS-Ø15Ø7-1 × SYN-Ø53Ø7-1
Developer(s)
Syngenta Seeds GmbH
Syngenta Seeds GmbH
Zum Knipkenbach 20
Bad Salzuflen
Germany, 32107
Phone:+49 52 22 5308-0
Fax:+49 52 22 5308-12
Email:info.seeds@syngenta.com
Url:Syngenta Seeds Germany
Description
The modified maize event was a result of cross-breeding modified parental lines and demonstrates herbicide tolerance and insect resistance. For Lepidoptera resistance, the maize expresses Bacillus thuringiensis Cry1Ab and Cry1F. For Coleoptera resistance, the maize expresses B. thuringiensis eCry3.1Ab. In addition to the insecticidal proteins, the maize also expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase for glufosinate tolerance. A selectable marker, Escherichia coli phosphomannose isomerase, is also present and was used during the transformation of the parental line using mannose selection.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
SYN-BTØ11-1 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
DAS-Ø15Ø7-1 - Herculex™ I maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
SYN-Ø53Ø7-1 - Agrisure® Duracade™ Maize
Mannose tolerance Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera), Northern corn rootworm (Diabrotica barberi) Selectable marker genes and reporter genes
Characteristics of the transformation process
Vector
pZO1502; PHI8999A; pSYN12274
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV 35S promoter
0.51 Kb
 
 
Alcohol Dehydrogenase 1, intron 6
0.47 Kb
 
 
Cry1Ab
1.85 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S promoter
0.42 Kb
 
 
Alcohol Dehydrogenase 1, intron 2
0.18 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ubiquitin gene promoter
0.98 Kb
 
 
Ubiquitin Intron 1
1.00 Kb
 
 
Cry1F
1.82 Kb
 
 
ORF25 PolyA Terminator sequence
0.72 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
 
Cestrum Yellow Leaf Curling Virus promoter
0.35 Kb
 
 
eCry3.1Ab
1.96 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from Bt11 vector pZO1502
Transcription of the Bacillus thuringiensis cry1Ab gene is under control of the Cauliflower Mosaic Virus (CaMV) 35S promoter and the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The transcript initially contains Zea mays alcohol dehydrogenase 1 intron 6, which enhance gene expression.

Transcription of the Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat) is under control of the CaMV 35S promoter and nos terminator. The transcript initially contains Zea mays alcohol dehydrogenase 1 intron 2 to enhance gene expression.

Note:
- The CaMV promoter associated with cry1Ab, was isolated from the CM1841 strain of CaMV using DdeI restriction digestion. However, the DdeI sites were converted into SacI sites.
- The CaMV promoter associated with pat was isolated from the Cabb-S strain of CaMV (AluI to DdeI fragment) and subsequently modified.
- The cry1Ab coding sequence encodes a truncated version corresponding to the N-terminal 615 amino acids of the full length Cry1Ab gene.
- The cloning of pat did not result in any amino acid sequence changes in the parental line.
- The nos terminator corresponds to the 423 to 678 basepairs of the nopaline synthase gene in A. tumefaciens.

DNA insert from TC1507 vector PHI8999A
DNA fragment PHI8999A contains two adjacent plant gene expression cassettes for Bacillus thuringiensis cry1F and Streptomyces viridochromogenes pat.

Transcription of cry1F is directed by the promoter and first exon and intron of the maize (Zea mays) ubiquitin gene and terminates at the Agrobacterium tumefaciens ORF25 terminator.

Transcription of the pat gene commences from the Cauliflower Mosaic Virus (CaMV) 35S promoter and ends at the CaMV 35S terminator.

Note:
- The coding sequence of both genes has been optimized to achieve a high level of expression in maize.
- The sequences of the complete cry1F and pat are identical to those in the original plasmid.
- The Cry1F protein includes the F604K (phenylalanine to lysine at position 604) amino acid substitution, which was introduced to create a specific restriction site for cloning purposes.

DNA insert from 5307 vector pSYN12274
The DNA insert contains two gene cassettes for an engineered chimeric protein eCry3.1Ab and an Escherichia coli phosphomannose isomerase (pmi).

Transcription of ecry3.1Ab is under control of a Cestrum Yellow Leaf Curling Virus promoter and an Agrobacterium tumefaciens nopaline synthase (nos) terminator. Transcription of pmi is under control of a Zea mays ubiquitin gene promoter and a nos terminator. The promoter contains the first intron of the ubiquitin gene, which will be initially included in the mRNA before splicing and for enhancing expression of pmi. Transcription is expected to be constitutive under both promoters and result in elevated levels of transgene expression.

Note:
- eCry3.1Ab is a result of a fusion of the 5′ end (Domain I, Domain II and 15 amino acids of Domain III) of a modified Cry3A gene (mcry3A) and the 3′ end (Domain III and Variable Region 6) of a synthetic Cry1Ab gene. The sequences were sourced from Bacillus thuringiesis.
- Southern blot analysis indicated that the parental line contains a single insertion of the vector and there was no integration of the vector backbone.
- Sequencing analysis indicated that the right and left T-DNA borders were truncated.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
  • Tolerance to mannose
Additional Information
Other relevant website address or attached documents