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Modified Organism
SPS-ØØØZ6-5 - Elevate potato
Record information and status
Record ID
115591
Status
Published
Date of creation
2020-05-29 19:28 UTC (austein.mcloughlin@cbd.int)
Date of last update
2020-05-29 19:31 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-05-29 19:31 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Elevate potato
Transformation event
Z6
Unique identifier
SPS-ØØØZ6-5
Developer(s)
J.R. Simplot Company
5369 West Irving Street 
Boise, ID
United States of America, 83706
Phone:+1 (208) 780-6066
Description
The Elevate potato (Event Z6) was created through the Agrobacterium-mediates transformation of the Invigorate potato (Event V11). The modified potato was modified for Phytophthora infestans resistance, reduced enzymatic blackening and low acrylamide potential. To achieve fungal resistance, the modified potato expresses a resistance gene from a wild potato, Solanum venturii (Rpi-vnt1). To reduce blackening of the potato tissue, an RNA interference (RNAi) cassette is expressed that targets the endogenous polyphenol oxidase-5 gene. To reduce the acrylamide potential, decrease levels of reducing sugars and asparagine were achieved through RNAi silencing of vacuolar invertase, water dikinase R1 and asparagine synthetase-1.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
SPS-ØØV11-6 - Innate® Invigorate Snowden
Changes in quality and/or metabolite content - Pigmentation / Coloration, Protein and amino acids
Point of collection or acquisition of the recipient organism
Z6 was produced through the transformation of V11 with vector pSIM1678.
V11 was produced through the transformation of potato variety Snowden with vector pSIM1278.
Characteristics of the transformation process
Vector
pSIM1278 and pSIM1678
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid left border repeat
0.19 Kb
 
 
ADP glucose pyrophosphorylase gene promoter
2.26 Kb
 
 
Asparagine synthetase-1 gene
0.41 Kb
 
 
Polyphenol oxidase 5 gene
0.14 Kb
 
 
Spacer sequence
0.16 Kb
 
 
Polyphenol oxidase 5 gene
0.14 Kb
 
 
Asparagine synthetase-1 gene
0.41 Kb
 
 
Granule bound starch synthase gene promoter
0.69 Kb
 
 
ADP glucose pyrophosphorylase gene promoter
2.26 Kb
 
 
Phosphorylase-L gene promoter
0.51 Kb
 
 
Alpha-glucan water dikinase R1 gene promoter
0.53 Kb
 
 
Spacer sequence
0.26 Kb
 
 
Alpha-glucan water dikinase R1 gene promoter
0.53 Kb
 
 
Phosphorylase-L gene promoter
0.51 Kb
 
 
Granule bound starch synthase gene promoter
0.92 Kb
 
 
Ti plasmid right border repeat
0.19 Kb
 
 
Ti plasmid left border repeat
0.19 Kb
 
 
Phytophthora infestans Resistance gene 1 Promoter
0.71 Kb
 
 
Phytophthora infestans Resistance gene 1
2.68 Kb
 
 
Phytophthora infestans Resistance gene 1 terminator
0.93 Kb
 
 
ADP glucose pyrophosphorylase gene promoter
2.26 Kb
 
 
Vacuolar invertase gene
0.50 Kb
 
 
Vacuolar invertase gene
0.18 Kb
 
 
Vacuolar invertase gene
0.50 Kb
 
 
Granule bound starch synthase gene promoter
0.92 Kb
 
 
Ti plasmid left border repeat
0.19 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from pSIM1278 (V11 genome)
The T-DNA insert contains two RNA interference (RNAi) cassettes for the potato genes: asparagine synthase-1 (Asn1) - polyphenol oxidase 5 (Ppo5) and phosphorylase-L (PhL) - Alpha-glucan water dikinase R1 (R1).

Asn1-Ppo5:
Transcription commences from both promoters, the potato ADP glucose pyrophosphorylase promoter (P-Agp) and the potato granule bound starch synthase promoter (P-Gbssp), which are in a convergent orientation relative to each other. The transcripts produced have inverted repeats of Asn1 and Ppo5 segments, separated by a spacer sequence derived from the potato genome. After transcription, the transcripts form double stranded RNA (dsRNA) molecules due to the homology of the sequences. The RNA molecules are then sufficient to trigger an RNAi response.

pPhL-R1:
Transcription is also directed from the convergent promoters P-Agp and P-Gbss. The RNA molecules contain inverted repeats of the promoter segments of PhL and R1, as well as a spacer derived from the potato genome. Post-transcription, the RNA molecules can form double stranded structures due to the sequence homology within the transcripts and trigger an RNAi response.

Note:
- The promoters are tuber-specific and expected to be most active in the tuber tissue.
- The transcripts are not expected to be translated into proteins.
- No specific terminators were added to the cassettes and thus the transcripts are not expected to have poly(A) tails.
- No marker genes (e.g. kanamycin or hygromycin resistance) are present.
- Southern blotting confirmed that the V11 genome contains a single T-DNA insertion.
- No vector backbone was incorporated into the V11 genome.

DNA insert from pSIM1678
The T-DNA insert contains two gene cassettes: Phytophora infestans resistance genes (Rpi-vnt1) from Solanum venturii and endogenous vacuolar invertase (vInv) RNAi.

Rpi-vnt1
Transcription of Rpi-vnt1 is under control of the native promoter and terminator.

vInv
Transcription is directed from the convergent promoters P-Agp and P-Gbss. The RNA molecules contain inverted repeats of the vInv, as well as a spacer, also derived from vInv. Post-transcription, the RNA molecules form double stranded structures due to the sequence homology within the transcripts and trigger an RNAi response.

Note:
- Southern blot analysis indicated that a single full length insertion of the T-DNA was inserted into the Z6 genome
LMO characteristics
Modified traits
  • Resistance to Phytophthora infestans
  • Reduced acrylamide potential
  • Reduced enzymatic browning
How the expression of the gene(s) was affected
Post-transcription, the sequence homology (inverted repeats) base pair to from hairpin RNA (hpRNA). The double strandedness of the hpRNA triggers an RNAi response. First, DICER recognizes the double stranded RNA of the hpRNA, associates with the hpRNA and fragments the hpRNA into ~21-23 basepair segments, termed small interfering RNAs (siRNA). ARGONAUTE family proteins bind the siRNA, unwind one of the strands to activate the RISC complex, which uses the remaining siRNA strand to target transcripts with sequence homology for degradation and thus results in gene silencing. Thus, the modified potato silences the gene expression of the following endogenous genes: Asn1, Ppo5, PhL, R1 and vInv.
Common use(s)
  • Food
Additional Information
Additional Information
Potatoes have the potential to contain acrylamide, a toxic/carcinogenic compound formed during exposure to high heat (i.e. during cooking). Acrylamide is formed through the Maillard reaction (non-enzymatic browning), where the carbonyl group of the  reducing sugar reacts with the nucleophilic amino group of the amino acid.