DNA insert from pSIM1278 (V11 genome)
The T-DNA insert contains two RNA interference (RNAi) cassettes for the potato genes: asparagine synthase-1 (Asn1) - polyphenol oxidase 5 (Ppo5) and phosphorylase-L (PhL) - Alpha-glucan water dikinase R1 (R1).

Asn1-Ppo5:
Transcription commences from both promoters, the potato ADP glucose pyrophosphorylase promoter (P-Agp) and the potato granule bound starch synthase promoter (P-Gbssp), which are in a convergent orientation relative to each other. The transcripts produced have inverted repeats of Asn1 and Ppo5 segments, separated by a spacer sequence derived from the potato genome. After transcription, the transcripts form double stranded RNA (dsRNA) molecules due to the homology of the sequences. The RNA molecules are then sufficient to trigger an RNAi response.

pPhL-R1:
Transcription is also directed from the convergent promoters P-Agp and P-Gbss. The RNA molecules contain inverted repeats of the promoter segments of PhL and R1, as well as a spacer derived from the potato genome. Post-transcription, the RNA molecules can form double stranded structures due to the sequence homology within the transcripts and trigger an RNAi response.

Note:
- The promoters are tuber-specific and expected to be most active in the tuber tissue.
- The transcripts are not expected to be translated into proteins.
- No specific terminators were added to the cassettes and thus the transcripts are not expected to have poly(A) tails.
- No marker genes (e.g. kanamycin or hygromycin resistance) are present.
- Southern blotting confirmed that the V11 genome contains a single T-DNA insertion.
- No vector backbone was incorporated into the V11 genome.

DNA insert from pSIM1678
The T-DNA insert contains two gene cassettes: Phytophora infestans resistance genes (Rpi-vnt1) from Solanum venturii and endogenous vacuolar invertase (vInv) RNAi.

Rpi-vnt1
Transcription of Rpi-vnt1 is under control of the native promoter and terminator.

vInv
Transcription is directed from the convergent promoters P-Agp and P-Gbss. The RNA molecules contain inverted repeats of the vInv, as well as a spacer, also derived from vInv. Post-transcription, the RNA molecules form double stranded structures due to the sequence homology within the transcripts and trigger an RNAi response.

Note:
- Southern blot analysis indicated that a single full length insertion of the T-DNA was inserted into the Z6 genome