Record details

Living Modified Organism identity

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LMO name

Insect resistant sugarcane

Transformation event

CTC91087

Unique identifier

CTC-91Ø87-6

Developer(s)

Record #112453

Dr Wladecir Salles Oliveira

Chairman of the Internal Biosafety Committee

CTC – Centro de Tecnologia Canavieira (CTC)

Fazenda Santo Antônio s/n, Caixa Postal 162

Piracicaba, Sao Paulo

Brazil, 13400-970

Phone:	+55 (11) 3429-8111
Fax:	+55 (11) 3429-8111
Email:	Wladecir.oliveira@ctc.com.br
Url:	CTC

Description

The sugarcane was modified for resistance to sugarcane borer (Diatraea saccharalis) through the expression of Bacillus thuringiensis Cry1Ac. The sugarcane additionally contains Streptomyces hygroscopicus phosphinothricin N-acetyltransferase gene as a selectable marker.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #115592

Saccharum officinarum L. - Sugarcane, Sugar cane

Point of collection or acquisition of the recipient organism

Sugarcane early variety CTC9001

Characteristics of the transformation process

Vector

p91087

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

Ubiquitin gene promoter

#100362

0.00 Kb

Cry1Ac

#14986

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

Ubiquitin gene promoter

#100362

0.00 Kb

Phosphinothricin N-acetyltransferase gene

#14972

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

Genetic cassettes:
The modified sugarcane contains two gene cassettes: Bacillus thuringiensis cry1Ac and Streptomyces hygroscopicus phosphothricin N-acetyltransferase (pat). Both gene cassettes are under transcriptional control of the Zea mays ubiquitin promoter and the Agrobacterium tumefaciens nopaline synthase terminator.

Notes:
- The coding sequence for cry1Ac was optimized for expression in sugarcane (sugarcane preferred codons).
- High levels of transcription are expected due to the constitutive nature of the ubiquitin promoter.

LMO characteristics

Modified traits

Resistance to diseases and pests
- Insects
  - Lepidoptera (butterflies and moths)
Resistance to herbicides
- Glufosinate
Selectable marker genes and reporter genes

Common use(s)

Food
Biofuel

Detection method(s)

External link(s)

The insect protected CTC91087 6 sugarcane event expresses Cry1Ac protein preferentially in leaves and presents compositional equivalence to.pdf

EUginius: CTC91087

Additional information

The Cry1Ac encodes a 615 amino acid protein with an estimated molecular weight of 68 kDa. The insecticidal core of the protein is 52 kDA.

DNA and protein on sugar samples produced from this modified sugarcane were unable to detection Cry1Ac or PAT (below the levels of detection) according Gianotto et al. (2019) (see attached document).

Additional Information

Other relevant website address or attached documents

CTC91087-6.pdf

EUginius - CTC91087

OECD BioTrack Database - CTC91087

ISAAA: CTC91087