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Record details
Modified Organism
CTC-91Ø87-6 - Insect resistant sugarcane
LMO Information
Decisions on the LMO
Risk Assessments
Record information and status
Record ID
115593
Status
Published
Date of creation
2020-05-29 20:01 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-05-29 20:01 UTC (austein.mcloughlin@cbd.int)
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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the
LMO quick-links
page.
LMO name
Insect resistant sugarcane
Transformation event
CTC91087
Unique identifier
CTC-91Ø87-6
Developer(s)
Record #112453
Dr Wladecir Salles Oliveira
Chairman of the Internal Biosafety Committee
CTC – Centro de Tecnologia Canavieira (CTC)
Fazenda Santo Antônio s/n, Caixa Postal 162
Piracicaba, Sao Paulo
Brazil, 13400-970
Phone:
+55 (11) 3429-8111
Fax:
+55 (11) 3429-8111
Email:
Wladecir.oliveira@ctc.com.br
Url:
CTC
Description
The sugarcane was modified for resistance to sugarcane borer (
Diatraea saccharalis
) through the expression of
Bacillus thuringiensis
Cry1Ac. The sugarcane additionally contains
Streptomyces hygroscopicus
phosphinothricin N-acetyltransferase gene as a selectable marker.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Record #115592
Saccharum officinarum L. - Sugarcane, Sugar cane
Point of collection or acquisition of the recipient organism
Sugarcane early variety CTC9001
Characteristics of the transformation process
Vector
p91087
Techniques used for the modification
Agrobacterium-mediated DNA transfer
Genetic elements construct
Ubiquitin gene promoter
#100362
0.00 Kb
Cry1Ac
#14986
0.00 Kb
Nopaline Synthase Gene Terminator
#100269
0.00 Kb
Ubiquitin gene promoter
#100362
0.00 Kb
Phosphinothricin N-acetyltransferase gene
#14972
0.00 Kb
Nopaline Synthase Gene Terminator
#100269
0.00 Kb
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Genetic cassettes:
The modified sugarcane contains two gene cassettes:
Bacillus thuringiensis
cry1Ac and
Streptomyces hygroscopicus
phosphothricin N-acetyltransferase (
pat
). Both gene cassettes are under transcriptional control of the
Zea mays
ubiquitin promoter and the
Agrobacterium tumefaciens
nopaline synthase terminator.
Notes:
- The coding sequence for
cry1Ac
was optimized for expression in sugarcane (sugarcane preferred codons).
- High levels of transcription are expected due to the constitutive nature of the ubiquitin promoter.
LMO characteristics
Modified traits
Resistance to diseases and pests
Insects
Lepidoptera (butterflies and moths)
Resistance to herbicides
Glufosinate
Selectable marker genes and reporter genes
Common use(s)
Food
Biofuel
Detection method(s)
External link(s)
The insect protected CTC91087 6 sugarcane event expresses Cry1Ac protein preferentially in leaves and presents compositional equivalence to.pdf
EUginius: CTC91087
Additional information
The Cry1Ac encodes a 615 amino acid protein with an estimated molecular weight of 68 kDa. The insecticidal core of the protein is 52 kDA.
DNA and protein on sugar samples produced from this modified sugarcane were unable to detection Cry1Ac or PAT (below the levels of detection) according Gianotto et al. (2019) (see attached document).
Additional Information
Other relevant website address or attached documents
CTC91087-6.pdf
EUginius - CTC91087
OECD BioTrack Database - CTC91087
ISAAA: CTC91087
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