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Modified Organism
Banana modified for Xanthomonas wilt disease resistance
Record information and status
Record ID
115602
Status
Published
Date of creation
2020-06-11 20:33 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-06-11 20:33 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Banana modified for Xanthomonas wilt disease resistance
Transformation event
HRAP
Developer(s)
Dr. Leena Tripathi
Principal Scientist
International Institute of Tropical Agriculture, Kenya (IITA)
Nairobi
Kenya
Phone:+254 208632900
Email:l.tripathi@cgiar.org
Description
The banana was modified for resistance to Xanthomonas wilt disease, caused by Xanthomonas campestris pv. musacearum through the expression of Capsicum annuum hypersensitive response-assisting protein. The modified banana plants demonstrate more a robust hypersensitive response upon infection with Gram negative bacteria, such as X. campestris, and thus resist  disease related wilting. The modified banana also contains a selectable marker, Ecscherichia coli neomycin phosphotransferase II, for kanamycin selection during transformation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Musa sp. - Banana, Plantain
Related LMOs
Banana resistant to Xanthomonas wilt disease
Dr. Leena Tripathi Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Bacteria
Show detection method(s)
Banana modified for Xanthomonas wilt disease resistance
Dr. Leena Tripathi Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Bacteria
Show detection method(s)
Characteristics of the transformation process
Vector
pBI-HRAP
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid right border repeat
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.18 Kb
 
 
Neomycin Phosphotransferase II
0.80 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S promoter
0.35 Kb
 
 
Hypersensitive response assisting protein gene
0.83 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ti plasmid left border repeat
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Gene expression
Transcription of Escherichia coli neomycin phosphotransferase II is under control of the Agrobacterium tumefaciens nopaline synthase (nos) promoter and terminator.

Transcription of Capsicum annuum hypersensitive response assisting protein (hrap) is under control of the Cauliflower Mosaic Virus 35S promoter and the nos terminator. High levels of transcription are expected due to the constitutive nature of the CaMV promoter.

Notes:
- The transformation vector was derived from the pBI121 vector using a BamHI and SacI restriction digest.
- The coding sequence of hrap was derived from the cDNA clone of the mRNA.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
  • Resistance to Xanthomonas sp.
Common use(s)
  • Food
  • Research
Detection method(s)
Additional information
Some detection methods can be found in the scientific article detailing the creation of this banana event.

Records referencing this document (2)
IDDescription
2record(s) found
Modified Organism2 records