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Modified Organism
Banana modified for Xanthomonas wilt disease resistance
Record information and status
Record ID
115603
Status
Published
Date of creation
2020-06-11 20:33 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-06-11 20:33 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Banana modified for Xanthomonas wilt disease resistance
Transformation event
PFLP
Developer(s)
Dr. Leena Tripathi
Principal Scientist
International Institute of Tropical Agriculture, Kenya (IITA)
Nairobi
Kenya
Phone:+254 208632900
Email:l.tripathi@cgiar.org
Description
The banana was modified for resistance to Xanthomonas wilt disease caused by Xanthomonas campestris pv. musacearum. The modified banana expresses Capsicum annuum plant ferredoxin-like protein (pflp), which intensifies the host plant's hypersensitive response triggered by invading bacterial pathogens and leads to an increased production of reactive oxygen species. The modified banana plants did not demonstrate wilting when challenged with X. campestris. In addition to pflp, the modified banana also contains a selectable marker, Escherichia coli neomycin phosphotransferase II, for kanamycin selection during transformation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Musa sp. - Banana, Plantain
Related LMOs
Banana resistant to Xanthomonas wilt disease
Dr. Leena Tripathi Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Bacteria
Show detection method(s)
Banana modified for Xanthomonas wilt disease resistance
Dr. Leena Tripathi Resistance to antibiotics - Kanamycin Resistance to diseases and pests
Show detection method(s)
Characteristics of the transformation process
Vector
pBI-PFLP
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid right border repeat
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.18 Kb
 
 
Neomycin Phosphotransferase II
0.80 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S promoter
0.35 Kb
 
 
Ferredoxin-like amphipathic protein
0.43 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ti plasmid left border repeat
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Gene expression
Transcription of Escherichia coli neomycin phosphotransferase II is under control of the Agrobacterium tumefaciens nopaline synthase (nos) promoter and terminator.

Transcription of Capsicum annuum plant ferredoxin-like protein (pflp) is under control of the Cauliflower Mosaic Virus (CaMV) 35S promoter and the nos terminator. Elevated levels transcription are expected due to the constitutive nature of the CaMV promoter.

Notes:
- The coding sequence of pflp is a cDNA copy of the natural transcript and thus introns are not present.
- PFLP contains an N-terminal peptide for localization to the chloroplasts of the cells. However, research has demonstrated that the transgenic protein may also be present in the cytoplasm.
- The transformation vector was derived from the pBI121 vector using a BamHI and SacI restriction digest.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
  • Resistance to Xanthomonas sp.
Common use(s)
  • Food
  • Research
Detection method(s)
Additional information
Some DNA and protein -based detection methodologies can be found in the scientific publication detailing the development of the banana event.

Records referencing this document (2)
IDDescription
2record(s) found
Modified Organism2 records