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Modified Organism
Banana resistant to Xanthomonas wilt disease
Record information and status
Record ID
115604
Status
Published
Date of creation
2020-06-11 20:33 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-06-11 20:33 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Banana resistant to Xanthomonas wilt disease
Transformation event
HRAP-PFLP
Developer(s)
Dr. Leena Tripathi
Principal Scientist
International Institute of Tropical Agriculture, Kenya (IITA)
Nairobi
Kenya
Phone:+254 208632900
Email:l.tripathi@cgiar.org
Description
The modified banana was a result of a stacked event and expresses Capsicum annuum plant ferredoxin-like protein and hypersensitive response-assisting protein for resistance to the bacterium Xanthomonas campestris pv. musacearum, the causal agent of Xanthomonas wilt disease. Both proteins intensify the hypersensitive response and increase reactive oxygen species in response to the pathogen associated molecular pattern, harpinPSS, and thus confer resistance to Gram negative bacterial pathogens, such as X. campestris. When challenged with X. campestris during glasshouse experiments, the modified banana plants did not exhibit wilting or signs of successful infection. In addition, the modified banana also contains a selectable marker, Escherichia coli neomycin phosphotransferase II, for kanamycin selection during transformation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Musa sp. - Banana, Plantain
Related LMOs
Banana modified for Xanthomonas wilt disease resistance
Dr. Leena Tripathi Resistance to antibiotics - Kanamycin Resistance to diseases and pests
Show detection method(s)
Banana modified for Xanthomonas wilt disease resistance
Dr. Leena Tripathi Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Bacteria
Show detection method(s)
Characteristics of the transformation process
Vector
pBI-HRAP-PFLP (derived from pBI121)
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid right border repeat
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.18 Kb
 
 
Neomycin Phosphotransferase II
0.80 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ferredoxin-like amphipathic protein
0.43 Kb
 
 
CaMV 35S promoter
0.35 Kb
 
 
CaMV 35S promoter
0.35 Kb
 
 
Hypersensitive response assisting protein gene
0.83 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ti plasmid left border repeat
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Gene expression
Transcription of Escherichia coli neomycin phosphotransferase II is under control of the Agrobacterium tumefaciens nopaline synthase (nos) promoter and terminator. (clockwise direction)

Transcription of Capsicum annuum plant ferredoxin-like protein (pflp) is under control of the Cauliflower Mosaic Virus (CaMV) 35S promoter and the nos terminator. (counterclockwise direction)

Transcription of C. annuum hypersensitive response assisting protein (hrap) is under control of the CaMV 35S promoter and the nos terminator. (clockwise direction)

Note:
- The coding sequence of pflp and hrap are cDNA copies of the natural transcript and thus introns are not present.
- Elevated levels of the Pflp and Hrap are expected due to the constitutive nature of the CaMV promoter.
- PFLP contains an N-terminal peptide for localization to the chloroplasts of the cells.
- The transformation vector was derived from the pBI-HRAP vector, which was obtained by using a BamHI and SacI restriction digest to remove the GUS reporter. A further HindIII digest allowed for the insertion of the pflp cassette in the opposite orientation.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
  • Resistance to Xanthomonas sp.
Common use(s)
  • Food
  • Research
Detection method(s)

Records referencing this document (3)
IDDescription
3record(s) found
Modified Organism2 records
Risk Assessment1 record