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Modified Organism
Cassava mosaic disease resistant cassava
Record information and status
Record ID
115623
Status
Published
Date of creation
2020-06-26 14:06 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-06-26 14:06 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Cassava mosaic disease resistant cassava
Transformation event
pCRNAi-dsCR
Developer(s)
Prof. Hassan Karakacha Were
Dean, School of Agriculture, Veterinary Science and Technology
Masinde Muliro University of Science and Technology (MMUST)
Kakamega
Kenya
Phone:(+254) (724) (972207)
Email:hwere@mmust.ac.ke
Description
The cassava was modified for resistance to Cassava mosaic disease (CMD) by introducing an RNA interference cassette that targets African cassava mosaic virus (ACMV) common region. The production of hairpin RNA by the host cells trigger an RNAi response that is expected to cause methylation of the viral DNA to prevent replication and/or expression of the viral genome. Due to conservation between CR sequences in ACMV and East african cassava mosaic virus, the modified cassava is expected to resistance to both viruses, which are causal agents of CMD. A selectable marker, Escherichia coli hygromycin B phosphotransferase, was additionally included for hygromycin selection during transformation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Manihot esculenta - Cassava, Brazilian arrowroot, Yuca, Manioc, Mandioca, MANES
Point of collection or acquisition of the recipient organism
Cassava cultivar TMS60444
Related LMOs
Cassava mosaic disease resistant cassava
Prof. Hassan Karakacha Were Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Viruses Selectable marker genes and reporter genes
Cassava mosaic disease resistant cassava
Prof. Hassan Karakacha Were Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Viruses Selectable marker genes and reporter genes
Cassava mosaic disease resistant cassava
Prof. Hassan Karakacha Were Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Viruses Selectable marker genes and reporter genes
Cassava brown streak disease resistant cassava
Prof. Hassan Karakacha Were Resistance to antibiotics - Hygromycin Selectable marker genes and reporter genes
Characteristics of the transformation process
Vector
pCAMBIA1300
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
Common region
0.26 Kb
 
 
M27939 Intron Sequence
0.20 Kb
 
 
Common region
0.26 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Hygromycin B phosphotransferase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
RNA interference cassette
The modified cassava contains an RNA interference (RNAi) cassette designed to target African cassava mosaic virus common region (CR). Transcription is initiated from the Cauliflower mosaic virus (CaMV) 35S promoter and terminates at the CaMV 35S terminator. The transcript contains two segments (sense and antisense) of CR separated by a plant synthetic intron. Post-transcription, the intron functions as a loop and allows the sense and antisense segments of CR base pair, forming hairpin RNA (hpRNA). The hpRNA acts as double stranded RNA (dsRNA), which triggers an RNAi response and the host cell machinery will target the CR region of the viral genome. Since the CR region is not transcribed, the RNAi response will not be directed towards viral transcripts. However, it is thought that the complementarity between the siRNA and the viral DNA triggers methylation to prevent replication and/or expression of the viral genome.

Note:
- The CR sequence corresponds to almost the entire sequence of the common region with core elements of the leftward promoter and the adjacent 86 bp sequence with core elements of the rightward promoter.
- The segment of CR corresponds to positions 21 to 277 of the ACMV DNA 1 (DNA A) (GenBank accession NC_001467).
- The source of the AC1 and AV1 sequences is ACMV strain West Kenyan 844.
- Due to the RNAi response, no proteins are expected to be translated from the RNAi cassette's transcript.

Selectable marker
Transcription of Escherichia coli hygromycin B phosphotransferase is under transcriptional control of the CaMV 35S promoter and Agrobacterium tumefaciens nopaline synthase terminator.
LMO characteristics
Modified traits
  • Resistance to African cassava mosaic virus
Common use(s)
  • Food

Records referencing this document (4)
IDDescription
4record(s) found
Modified Organism4 records