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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide tolerant, Insect resistant maize
EN
MON87427 × MIR162
Yes
MON-87427-7 × SYN-IR162-4
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Person:Bayer CropScienceBayer CropScience AG Alfred-Nobel-Str. 50 40789 Monheim am RheinMonheim am Rhein,
40789, GermanyPhone: +49 21 73 - 38-0,Fax:Email:Related OrganizationBayer CropScience Deutschland GmbH ()Private sector (business and industry)Bayer CropScience AG Alfred-Nobel-Str. 50 40789 Monheim am RheinMonheim am Rhein,
40789, GermanyPhone: +49 21 73 - 38-0,Fax:Email:
The modified maize was produced through the cross breeding of two modified parental lines for herbicide tolerance and insect resistance. For Lepidoptera resistance, the maize expresses Bacillus thuringiensis Vegetative insecticidal protein 3Aa20. For herbicide tolerance, the maize expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase, which encodes a variant of the endogenous enzyme involved in aromatic amino acid synthesis and confers glyphosate tolerance in female and vegetative tissues. The tissue specificity additionally allows for glyphosate-treated plants to serve as a female parent in the production of hybrid lines. The modified maize also contains a selectable, Escherichia coli phosphomannose isomerase, for mannose selection during parental transformation.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
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BCH-LMO-SCBD-104758-3 Living Modified Organism MON-87427-7 - Maize modified for tissue selective glyphosate toleranceMonsanto | Resistance to herbicides (Glyphosate)
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BCH-LMO-SCBD-100885-13 Living Modified Organism SYN-IR162-4 - Agrisure™ Viptera maizeResistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
EN
PV-ZMAP1043; pNOV1300
EN
- Cross breeding
0.620 kb
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0.800 kb
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0.230 kb
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1.370 kb
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0.250 kb
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1.990 kb
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2.370 kb
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0.110 kb
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0.070 kb
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1.990 kb
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1.180 kb
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0.250 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-100887-5 Vegetative insecticidal protein 3Aa20 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-101406-4 Phosphoenolpyruvate carboxylase, intron 9 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-15003-7 Phosphomannose Isomerase gene | Escherichia coli (ECOLX)Protein coding sequence | Mannose tolerance,Selectable marker genes and reporter genes
Genetic elements from PV-ZMAP1043
Transcription of 5-enolpyruvylshikimate-3-phosphate synthase (cp4 epsps) from Agrobacterium tumefaciens commences from the Cauliflower mosaic virus (CaMV) enhanced 35S promoter and ends at the A. tumefaciens nopaline synthase (nos) gene terminator. The transcript contains a Zea mays heat shock protein 70 (Hsp70) intron, Arabidopsis thaliana N-terminal chloroplast transit peptide sequence, and cp4 epsps. The CaMV enhanced 35S promoter-hsp70 combination promotes gene expression in female and vegetative tissues, but not in male reproductive tissues (pollen microspores and tapetum).
Note:
- Southern blot analyses indicate that a single copy of the T-DNA was inserted at a single site in the parental maize genome and no plasmid vector backbone sequences were detected to have been integrated. DNA sequencing analyses further indicated that the expected T-DNA sequences were integrated.
-The cp4 epsps coding sequence is the codon optimized coding sequence of the aroA gene from Agrobacterium sp. strain CP4 encoding CP4 EPSPS.
Genetic elements from pNOV1300
In the parental MIR162 maize, a variant of the native B. thuringiensis vegetative insecticidal protein 3Aa (vip3Aa20), named vip3Aa19, which has codon changes that result in a single M129I amino acid substitution was inserted into the transformation cassette. During the transformation process an additional DNA mutation resulted in a K284Q amino acid substitution. This final form was designated the name Vip3Aa20. Transcription of vip3Aa20 commences at the Z. mays ubiquitin gene promoter and then transcribes vip3Aa20 followed by intron 9 of Z. mays phosphoenolpyruvate carboxylase, before terminating at the CaMV 35S terminator.
A second expression cassette, containing the E. coli phosphomannose isomerase gene, was also inserted into the parental genome. The gene is under the control of another ubiquitin promoter and transcription terminates at the Agrobacterium tumefaciens nopaline synthase gene (nos) terminator.
Note:
- Southern blot analyses demonstrated that the T-DNA insert contains: i) single copies of a vip3Aa20 gene and a pmi gene; ii) two copies of the ZmUbiInt promoter; iii) one copy of the nos terminator; and iv) no backbone sequences from transformation plasmid pNOV1300.
EN
Transcription of 5-enolpyruvylshikimate-3-phosphate synthase (cp4 epsps) from Agrobacterium tumefaciens commences from the Cauliflower mosaic virus (CaMV) enhanced 35S promoter and ends at the A. tumefaciens nopaline synthase (nos) gene terminator. The transcript contains a Zea mays heat shock protein 70 (Hsp70) intron, Arabidopsis thaliana N-terminal chloroplast transit peptide sequence, and cp4 epsps. The CaMV enhanced 35S promoter-hsp70 combination promotes gene expression in female and vegetative tissues, but not in male reproductive tissues (pollen microspores and tapetum).
Note:
- Southern blot analyses indicate that a single copy of the T-DNA was inserted at a single site in the parental maize genome and no plasmid vector backbone sequences were detected to have been integrated. DNA sequencing analyses further indicated that the expected T-DNA sequences were integrated.
-The cp4 epsps coding sequence is the codon optimized coding sequence of the aroA gene from Agrobacterium sp. strain CP4 encoding CP4 EPSPS.
Genetic elements from pNOV1300
In the parental MIR162 maize, a variant of the native B. thuringiensis vegetative insecticidal protein 3Aa (vip3Aa20), named vip3Aa19, which has codon changes that result in a single M129I amino acid substitution was inserted into the transformation cassette. During the transformation process an additional DNA mutation resulted in a K284Q amino acid substitution. This final form was designated the name Vip3Aa20. Transcription of vip3Aa20 commences at the Z. mays ubiquitin gene promoter and then transcribes vip3Aa20 followed by intron 9 of Z. mays phosphoenolpyruvate carboxylase, before terminating at the CaMV 35S terminator.
A second expression cassette, containing the E. coli phosphomannose isomerase gene, was also inserted into the parental genome. The gene is under the control of another ubiquitin promoter and transcription terminates at the Agrobacterium tumefaciens nopaline synthase gene (nos) terminator.
Note:
- Southern blot analyses demonstrated that the T-DNA insert contains: i) single copies of a vip3Aa20 gene and a pmi gene; ii) two copies of the ZmUbiInt promoter; iii) one copy of the nos terminator; and iv) no backbone sequences from transformation plasmid pNOV1300.
EN
- Food
- Feed
- MON-87427-7 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- SYN-IR162-4 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- MON-87427-7 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-IR162-4 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-87427-7 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- SYN-IR162-4 - CropLife International Detection Methods Database ( CropLife ) [ English ]
EN
EN
- EUginius - MON87427 x MIR162 [ English ]
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