The maltose binding protein (MBP) purification tag is an amino acid
sequence attached proteins to allow for the purification and
isolation of a recombinant using amylose resin-based
chromatography. The methodology exploits the maltose binding
capabilities of Escherichia coli gene malE
(maltose/maltodextrin-binding periplasmic protein). During
purification, the MBP tag binds to amylose resin while the other,
non-tagged proteins are eluted. Next, malltose is added to release
the MBP-tagged protein from the resin.
The MPB tag improves solubility and reduces toxicity of the
recombinant protein compared to other affinity tags. However, the
MBP tag is larger than other affinity tags and can potentially be
more allergenic.
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