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Modified Organism
MON-87427-7 × MON-ØØ81Ø-6 - Herbicide tolerant, insect resistant maize
Record information and status
Record ID
115718
Status
Published
Date of creation
2020-09-10 19:13 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-09-10 19:13 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide tolerant, insect resistant maize
Transformation event
MON87427 × MON810
Unique identifier
MON-87427-7 × MON-ØØ81Ø-6
Developer(s)
Description
The modified maize (Zea mays) was produced through cross breeding of modified parental lines to express tolerance to herbicides and resistance to insects. For Lepidoptera resistance, the modified maize expresses Bacillus thuringiensis Cry1Ab, commonly known as "Bt toxin". The protein forms pores in the midgut lining of susceptible pests, leading to cell lysis and septicemia. For glyphosate tolerance, the maize expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase, which encodes a bacterial variant of an endogenous enzyme involved in the essential biosynthesis of aromatic amino acids (shikimate pathway). The  bacterial protein does not bind the herbicidal compound with high affinity and thus prevents inactivation of the enzyme.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
MON-87427-7 - Maize modified for tissue selective glyphosate tolerance
Resistance to herbicides - Glyphosate
Show detection method(s)
MON-ØØ81Ø-6 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
Characteristics of the transformation process
Vector
PV-ZMAP1043; PV-ZMBK07 and PV-ZMGT10
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Cry1Ab
3.46 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from MON87427 (MON-87427-7) vector PV-ZMAP1043
Transcription of Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (epsps) commences from the Cauliflower mosaic virus (CaMV) enhanced 35S promoter and terminates at the A. tumefaciens nopaline synthase (nos) terminator. The transcript contains a Zea mays heat shock protein 70 (hsp70) intron, an Arabidopsis thaliana N-terminal chloroplast transit peptide sequence for chloroplast targeting of the protein and epsps. The CaMV enhanced 35S promoter-hsp70 combination promotes gene expression in female and vegetative tissues, but not in male reproductive tissues (pollen microspores and tapetum).

Note:
- Southern blot analyses indicate that a single copy of the T-DNA was inserted at a single site in the parental maize genome and no plasmid vector backbone sequences were detected to have been integrated. DNA sequencing analyses further indicated that the expected T-DNA sequences were integrated.
-The epsps coding sequence is the codon optimized coding sequence of the aroA gene from Agrobacterium sp. strain CP4 encoding EPSPS.

DNA insert from MON810 (MON-ØØ81Ø-6) vector PV-ZMBK07
A partial insert containing Bacillus thuringiensis cry1Ab was inserted into the parental maize genome. Transcription is directed from the Cauliflower mosaic virus 35S enhanced promoter. The transcript contains a Zea mays heat shock protein 70 (ZmHsp70) intron and the coding sequence of cry1Ab. ZmHsp70 enhances expression of cry1Ab.

Note:
- The coding sequence of cry1Ab has been codon optimized for expression in plants. The codon optimization did not result in any changes to the amino acid sequence relative to the native sequence.
- Southern blot analysis indicated that a single partial insert is found within the parental genome.
- Southern blot analysis did not detect the presence of the Escherichia coli neomycin phosphotransferase II gene nor any DNA from plasmid PVZMGT10 (containing genes for glyphosate tolerance - epsps).
- ELISA protein analysis and feeding assays indicated expression of Cry1Ab.

Kindly refer to the parental LMO records for more information.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents