Record details

Living Modified Organism identity

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LMO name

Moonstrike™ carnation

Transformation event

406851

Unique identifier

FLO-4Ø686-3

Developer(s)

Record #4953

Stephen Chandler

Cosnultant

SUntory Holdings Ltd.

Melbourne, VIC

Australia

Phone:	+61 409 387 386
Email:	schandler@florigene.com.au
Url:	Florigene Homepage

Description

The modified carnation (Dianthus caryophyllus) was derived from the parental Moonvista™ carnation through vegetative propagation. The Moonstrike™ carnation differs from the parental variety in having a flecked, bi-colour flower colour pattern. The modified carnation contains Petunia hybrida dihydroxflavonol-4 reductase and Viola sp. flavonoid 3' 5' hydroxylase, which together promoter biosynthesis of delphinidin and anthocyanin pigments. The pigment bioproduction results in petals that are eggplant purple in colour. The modified carnation additionally contains Nicotiania tabacum acetolactate synthase for chlorsulfuron selection during tissue culturing.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #4954

Dianthus caryophyllus - Carnation, DIACA

Record #14835

FLO-4Ø685-2 - Moonvista™ carnation

Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea

Show detection method(s)

Point of collection or acquisition of the recipient organism

Moonstrike™ is a clone of the carnation variety FLORIGENE Moonvista™.

Related LMOs

Record #115777

FLO-4Ø62Ø-9 - Moonburst™ carnation

Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea

Characteristics of the transformation process

Vector

pCGP1991

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

CaMV 35S promoter

#100287

0.19 Kb

5' untranslated leader of chlorophyll a/b-binding protein

#101901

0.08 Kb

Acetohydroxy acid synthase gene

#15177

3.76 Kb

Acetohydroxy acid synthase gene terminator

#100390

0.00 Kb

Dihydroflavonol-4-reductase promoter

#105798

0.00 Kb

Dihydroflavonol-4-reductase

#15009

4.96 Kb

Dihydroflavonol-4-reductase terminator

#105799

0.00 Kb

Chalcone synthase gene promoter

#103771

1.16 Kb

Flavonoid 3’, 5’-hydroxylase gene

#43793

1.78 Kb

D8 gene terminator

#103772

0.82 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

Gene expression
Three gene cassettes are present: Nicotiania tabacum acetolactate synthase (ALS; acetohydroxy acid synthase), Petunia hybrida dihydroflavonol-4-reductase (DFR) and Viola sp. flavonoid3', 5'-hydroxylase (F3'5'H).

Transcription of ALS is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and a N. tabacum ALS terminator. A 5' untranslated leader sequence from P. hybrida chlorophyll a/b-binding protein is also present at the 5' end of ALS, but is not expected to be translated. The leader sequence promotes high levels of transcription of ALS.

Transcription of DFR is under control of its endogenous promoter and terminator. The coding sequence contains 6 exons and 5 introns.

Transcription of F3'5'H is under control of an Antirrhinum majus chalcone synthase promoter and a P. hybrida D8 terminator.

Note:
- The size of the ALS coding sequence includes the size of the terminator (3.76 kb = size of ALS coding sequence + ALS terminator)
- The size of the DFR coding sequence represents the size of the full genomic cone (4.96 kb = DFR promoter + DFR coding sequence + DFR terminator)

For more information, kindly refer to the parental record.

LMO characteristics

Modified traits