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Modified Organism
FLO-4Ø686-3 - Moonstrike™ carnation
Record information and status
Record ID
115776
Status
Published
Date of creation
2020-11-23 15:03 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-11-23 15:03 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Moonstrike™ carnation
Transformation event
406851
Unique identifier
FLO-4Ø686-3
Developer(s)
Stephen Chandler
Cosnultant
SUntory Holdings Ltd.
Melbourne, VIC
Australia
Phone:+61 409 387 386
Email:schandler@florigene.com.au
Url:Florigene Homepage
Description
The modified carnation (Dianthus caryophyllus) was derived from the parental Moonvista™ carnation through vegetative propagation. The Moonstrike™ carnation differs from the parental variety in having a flecked, bi-colour flower colour pattern. The modified carnation contains  Petunia hybrida dihydroxflavonol-4 reductase and Viola sp. flavonoid 3' 5' hydroxylase, which together promoter biosynthesis of delphinidin and anthocyanin pigments. The pigment bioproduction results in petals that are eggplant purple in colour. The modified carnation additionally contains Nicotiania tabacum acetolactate synthase for chlorsulfuron selection during tissue culturing.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Dianthus caryophyllus - Carnation, DIACA
FLO-4Ø685-2 - Moonvista™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
Show detection method(s)
Point of collection or acquisition of the recipient organism
Moonstrike™ is a clone of the carnation variety FLORIGENE Moonvista™.
Related LMOs
FLO-4Ø62Ø-9 - Moonburst™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
Characteristics of the transformation process
Vector
pCGP1991
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.19 Kb
 
 
5' untranslated leader of chlorophyll a/b-binding protein
0.08 Kb
 
 
Acetohydroxy acid synthase gene
3.76 Kb
 
 
Acetohydroxy acid synthase gene terminator
0.00 Kb
 
 
Dihydroflavonol-4-reductase promoter
0.00 Kb
 
 
Dihydroflavonol-4-reductase
4.96 Kb
 
 
Dihydroflavonol-4-reductase terminator
0.00 Kb
 
 
Chalcone synthase gene promoter
1.16 Kb
 
 
Flavonoid 3’, 5’-hydroxylase gene
1.78 Kb
 
 
D8 gene terminator
0.82 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Gene expression
Three gene cassettes are present: Nicotiania tabacum acetolactate synthase (ALS; acetohydroxy acid synthase), Petunia hybrida dihydroflavonol-4-reductase (DFR) and Viola sp. flavonoid3', 5'-hydroxylase (F3'5'H).

Transcription of ALS is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and a N. tabacum ALS terminator. A 5' untranslated leader sequence from P. hybrida chlorophyll a/b-binding protein is also present at the 5' end of ALS, but is not expected to be translated. The leader sequence promotes high levels of transcription of ALS.

Transcription of DFR is under control of its endogenous promoter and terminator. The coding sequence contains 6 exons and 5 introns.

Transcription of F3'5'H is under control of an Antirrhinum majus chalcone synthase promoter and a P. hybrida D8 terminator.

Note:
- The size of the ALS coding sequence includes the size of the terminator (3.76 kb = size of ALS coding sequence + ALS terminator)
-  The size of the DFR coding sequence represents the size of the full genomic cone (4.96 kb = DFR promoter + DFR coding sequence + DFR terminator)

For more information, kindly refer to the parental record.
LMO characteristics
Modified traits
Common use(s)
  • Ornamental
Detection method(s)
Additional information
Note: The detection methods of the parental variety are shown and expected to be applicable to this variety as well.

Records referencing this document (1)
IDDescription
1record(s) found
Modified Organism1 record