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Modified Organism
FLO-4Ø62Ø-9 - Moonburst™ carnation
Record information and status
Record ID
115777
Status
Published
Date of creation
2020-11-23 15:03 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-11-23 15:03 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Moonburst™ carnation
Transformation event
406191
Unique identifier
FLO-4Ø62Ø-9
Developer(s)
Stephen Chandler
Cosnultant
SUntory Holdings Ltd.
Melbourne, VIC
Australia
Phone:+61 409 387 386
Email:schandler@florigene.com.au
Url:Florigene Homepage
Description
The modified carnation (Dianthus caryophyllus) was vegetatively propagated from the parental variety Moonshade™ and  differs from the parent in having a flecked, bi-colour flower colour pattern. The carnation contains Petunia hybrida flavonoid 3'5' hydroxylase and dihydroflavonol-4 reductase, which together promote biosynthesis of delphinidin and anthocyanin pigments. The accumulation of pigments results in lavendar-coloured flowers. The modified carnation additionally contains Nicotiania tabacum acetolactate synthase for chlorsulfuron selection during tissue culturing.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Dianthus caryophyllus - Carnation, DIACA
FLO-4Ø619-8 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea Selectable marker genes and reporter genes
Point of collection or acquisition of the recipient organism
Moonburst™ carnation is a clone of the FLORIGENE Moonshade™ carnation.

The parental Moonshade carnation was sourced from the "UNESCO White" variety (cultivar FE 123), which contains a mutation in DFR and results in a non-functional enzyme (resulting in white flowers).
Related LMOs
FLO-4Ø686-3 - Moonstrike™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
Show detection method(s)
Characteristics of the transformation process
Vector
pCGP1470
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.19 Kb
 
 
5' untranslated leader of chlorophyll a/b-binding protein
0.06 Kb
 
 
Acetohydroxy acid synthase gene
3.76 Kb
 
 
Acetohydroxy acid synthase gene terminator
0.00 Kb
 
 
Chalcone synthase gene promoter
1.16 Kb
 
 
Flavonoid 3', 5' hydroxylase gene
1.52 Kb
 
 
D8 gene terminator
0.82 Kb
 
 
mac-1 Promoter
1.23 Kb
 
 
Dihydroflavonol-4-reductase
1.14 Kb
 
 
Mannopine synthase gene terminator
0.72 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Gene expression
The T-DNA insertion contained three gene cassettes: Nicotiania tabacum acetolactate synthase (ALS; acetohydroxy acid synthase), Petunia hybrida flavonoid 3', 5' hydroxylase (F3'5'H) and P. hybrida dihydroflavonol-4-reductase (DFR).

Transcription of ALS is under control of the Cauliflower mosaic virus (CaMV) 35S promoter and the ALS terminator. A 5' untranslated leader from P. hybrida chlorophyll a/b-binding protein is also transcribed, but is not expected to be translated. The leader sequence augments the level of transcription (enhances the expression) of ALS.

Transcription of F3'5'H is under control of the Antirrhinum majus chalcone synthase gene promoter and the P. hybrida D8 terminator.

Transcription of DFR is under control of the synthetic Mac-1 promoter and the Agrobacterium tumefaciens mannopine synthase gene terminator.

Note:
- The genetic element size of the ALS coding sequence includes the size of the terminator (3.76 kb = size of coding sequence + terminator)
- The Mac-1 promoter is a synthetic promoter compromised of A. tumefaciensmas promoter and CaMV enhancer sequences.

For more information, kindly refer to the parental record.
LMO characteristics
Modified traits
Common use(s)
  • Ornamental

Records referencing this document (2)
IDDescription
2record(s) found
Modified Organism2 records