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Record details
Modified Organism
FLO-4Ø62Ø-9 - Moonburst™ carnation
LMO Information
Decisions on the LMO
Risk Assessments
Record information and status
Record ID
115777
Status
Published
Date of creation
2020-11-23 15:03 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-11-23 15:03 UTC (austein.mcloughlin@cbd.int)
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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the
LMO quick-links
page.
LMO name
Moonburst™ carnation
Transformation event
406191
Unique identifier
FLO-4Ø62Ø-9
Developer(s)
Record #4953
Stephen Chandler
Cosnultant
SUntory Holdings Ltd.
Melbourne, VIC
Australia
Phone:
+61 409 387 386
Email:
schandler@florigene.com.au
Url:
Florigene Homepage
Description
The modified carnation (
Dianthus caryophyllus
) was vegetatively propagated from the parental variety Moonshade™ and differs from the parent in having a flecked, bi-colour flower colour pattern. The carnation contains
Petunia hybrida
flavonoid 3'5' hydroxylase and dihydroflavonol-4 reductase, which together promote biosynthesis of delphinidin and anthocyanin pigments. The accumulation of pigments results in lavendar-coloured flowers. The modified carnation additionally contains
Nicotiania tabacum
acetolactate synthase for chlorsulfuron selection during tissue culturing.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Record #4954
Dianthus caryophyllus - Carnation, DIACA
Record #14828
FLO-4Ø619-8 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea Selectable marker genes and reporter genes
Point of collection or acquisition of the recipient organism
Moonburst™ carnation is a clone of the FLORIGENE Moonshade™ carnation.
The parental Moonshade carnation was sourced from the "UNESCO White" variety (cultivar FE 123), which contains a mutation in DFR and results in a non-functional enzyme (resulting in white flowers).
Related LMOs
Record #115776
FLO-4Ø686-3 - Moonstrike™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
Show detection method(s)
Characteristics of the transformation process
Vector
pCGP1470
Techniques used for the modification
Agrobacterium-mediated DNA transfer
Genetic elements construct
CaMV 35S promoter
#100287
0.19 Kb
5' untranslated leader of chlorophyll a/b-binding protein
#101901
0.06 Kb
Acetohydroxy acid synthase gene
#15177
3.76 Kb
Acetohydroxy acid synthase gene terminator
#100390
0.00 Kb
Chalcone synthase gene promoter
#103771
1.16 Kb
Flavonoid 3', 5' hydroxylase gene
#15010
1.52 Kb
D8 gene terminator
#103772
0.82 Kb
mac-1 Promoter
#103773
1.23 Kb
Dihydroflavonol-4-reductase
#15009
1.14 Kb
Mannopine synthase gene terminator
#103774
0.72 Kb
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Gene expression
The T-DNA insertion contained three gene cassettes:
Nicotiania tabacum
acetolactate synthase (ALS; acetohydroxy acid synthase),
Petunia hybrida
flavonoid 3', 5' hydroxylase (F3'5'H) and
P. hybrida
dihydroflavonol-4-reductase (DFR).
Transcription of ALS is under control of the
Cauliflower mosaic virus
(CaMV) 35S promoter and the ALS terminator. A 5' untranslated leader from
P. hybrida
chlorophyll a/b-binding protein is also transcribed, but is not expected to be translated. The leader sequence augments the level of transcription (enhances the expression) of ALS.
Transcription of F3'5'H is under control of the
Antirrhinum majus
chalcone synthase gene promoter and the
P. hybrida
D8 terminator.
Transcription of DFR is under control of the synthetic Mac-1 promoter and the
Agrobacterium tumefaciens
mannopine synthase gene terminator.
Note:
- The genetic element size of the ALS coding sequence includes the size of the terminator (3.76 kb = size of coding sequence + terminator)
- The Mac-1 promoter is a synthetic promoter compromised of
A. tumefaciens
mas
promoter and CaMV enhancer sequences.
For more information, kindly refer to the parental record.
LMO characteristics
Modified traits
Changes in quality and/or metabolite content
Pigmentation / Coloration
Resistance to herbicides
Chlorsulfuron
Sulfonylurea
Common use(s)
Ornamental
Additional Information
Other relevant website address or attached documents
FLORIGENE® Moonburst™
Pigment biosynthesis in flowers.docx
Flower colour and cytochromes P450.pdf
EUginius - FLO-4Ø619-8 (parental variety)
Records referencing this document
(
2
)
ID
Description
2
record(s) found
Modified Organism
2 records
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