R-2,4-dichlorophenoxypropionate dioxygenase

Rdpa

CS-ft_t-SPHHE

The protein sequence of (R)-dichlorprop dioxygenase was used as a scaffold in the engineering of the protein.

The Sphingobium herbicidovorans R-2,4-dichlorophenoxypropionate dioxygenase was engineered to have increased thermostability and enzymatic activity. The enzyme inactivates quizalofop (active against monocots) and 2,4-dichlorophenoxyacetic acid herbicides using α-ketoglutarate and molecular oxygen to produce the inactivated herbicide, succinate and carbon dioxide. The enzyme may also act on other aryloxyphenoxypropionate and synthetic auxin herbicides.