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Modified Organism
MAH-EEØØ1-6 - Bt eggplant (Bt brinjal)
Record information and status
Record ID
115888
Status
Published
Date of creation
2021-02-16 22:01 UTC (austein.mcloughlin@cbd.int)
Date of last update
2021-09-14 14:50 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-09-14 14:50 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Bt eggplant (Bt brinjal)
Transformation event
EE-1
Unique identifier
MAH-EEØØ1-6
Developer(s)
Maharashtra Hybrid Seeds Company Limited (Mahyco)
Head Office
Jalna - Aurangabad Road,
Post Box no. 76,
Dawalwadi, Jalna
Dawalwadi, Maharashtra
India, 431203
Phone:+91 02482-262471
Fax:+91 02482-26002
Email:info@mahyco.com
Url:Mahyco Private Limited
Description
The eggplant (brinjal, aubergine; Solanum melongena) has been modified for resistance to Lepidoptera pests (Eggplant Fruit and Shoot Borer) through the expression of a Bacillus thuringiensis crystal protein Cry1Ac. The protein forms pores in the midgut lining of susceptible pests, leading to cell lysis and septicemia. For selection during transformation of the eggplant, an Escherichia coli neomycin phosphotransferase II cassette was inserted for kanamycin resistance. Additionally, a bacterial gene cassette, E. coli  3''(9)-O-aminoglycoside adenyltransferase, is also present and was used for streptomycin/spectromycin selection during cloning.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum melongena - Eggplant, Aubergine, Brinjal
Point of collection or acquisition of the recipient organism
See note in 'Additional information' section.
Characteristics of the transformation process
Vector
pMON10518
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
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Neomycin Phosphotransferase II
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Nopaline Synthase Gene Terminator
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3"(9)-O-aminoglycoside adenyltransferase
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CaMV Enhanced 35S promoter
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Cry1Ac
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α' subunit of β-conglycinin gene terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The modified eggplants contains three cassetes: Escherichia coli neomycin phosphotransferase (nptII), E. coli 3''(9)-O-aminoglycoside adenyltransferase (aadA) and Bacillus thuringiensis cry1Ac.

The nptII cassette is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an Agrobacterium tumefaciens nopaline synthase (nos) terminator.

The aadA coding sequence is under control of the endogenous, bacterial promoter and terminator.

The cry1Ac cassette is under control of the CaMV 35S enhanced promoter and Glycine max 3' region encoding the 7S alpha seed storage protein (α' subunit of β-conglycinin) terminator.

Note:
- Transcription of nptII and cry1Ac are expected to occur at elevated levels due to the CaMV promoters.
- Transcription of aadA is not expected in the host eggplant because the regulatory elements are only active in bacteria (i.e. E. coli).
- Please refer to 'Additional information' section regarding introgression of genetic cassettes into other varieties.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Food
Additional Information
Additional Information
To create the event EE-1, the transgene constructs were initially introduced into brinjal line 60208, which has purple variegated, oval fruit (see patent). However, it is important to note that EE-1 has been introgressed into (hybridized with) other varieties of eggplant through open pollination. Thus, the modified eggplant may have different names and phenotypes. Examples include:
- Bt Uttara (BARI Bt Brinjal-1): long, purple-coloured, cylindrical fruits; plants are bushy
- Bt Kajla (BARI Bt Brinjal-2): long, black-purple, cylindrical fruits
- Bt Nayantara (BARI Bt Brinjal-3): round, dark purple fruits
- Bt Iswardi or ISD006 (BARI Bt Brinjal-4): light green oblong fruits