Record details

Living Modified Organism identity

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LMO name

Bt okra

Transformation event

OE-17A

Developer(s)

Record #115887

Maharashtra Hybrid Seeds Company Limited (Mahyco)

Head Office

Jalna - Aurangabad Road,
Post Box no. 76,
Dawalwadi, Jalna

Dawalwadi, Maharashtra

India, 431203

Phone:	+91 02482-262471
Fax:	+91 02482-26002
Email:	info@mahyco.com
Url:	Mahyco Private Limited

Description

The okra (Abelmoschus esculentus) was modified for resistance to Lepidoptera pests (e.g. spotted bollworm) through the expression of Bacillus thuringiensis Cry1Ac. The protein forms pores in the midgut lining of susceptible pests, leading to cell lysis and septicemia. Additionally, the okra contains an Escherichia coli neomycin phosphotransferase II cassette for kanamycin selection during transformation.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #115629

Abelmoschus esculentus - Okra, Lady's finger

Characteristics of the transformation process

Vector

pC2300cry1Ac10518ve

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

CaMV Enhanced 35S promoter

#100366

0.00 Kb

Cry1Ac

#14986

0.00 Kb

transcription stop

(sense)

CaMV 35S promoter

#100287

0.00 Kb

Neomycin Phosphotransferase II

#15001

0.00 Kb

CaMV 35S terminator

#100290

0.00 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

The modified okra includes two gene cassettes: Bacillus thuringiensis cry1Ac and Escherichia coli neomycin phosphotransferase II (nptII).

The cry1Ac is under control of the Cauliflower mosaic virus (CaMV) 35S enhanced promoter and a poly(A) terminator. Due to the constitutive nature of the promoter, transcription is expected to occur at high levels.

Transcription of nptII is under control of the CaMV 35S promoter and 35S terminator. Again, transcription is expected to occur at high levels due to the nature of the viral promoter.

LMO characteristics

Modified traits

Resistance to antibiotics
- Kanamycin
Resistance to diseases and pests
- Insects
  - Lepidoptera (butterflies and moths)
Selectable marker genes and reporter genes

Common use(s)

Food

Additional Information

Other relevant website address or attached documents

Transformation vector.pdf

Description from Bt okra from provisional patent.pdf

Sequences - provisional patent.pdf

Efficient genetic transformation of okra (Abelmoschus esculentus (L.) Moench) and generation of insect-resistant transgenic plants expressing the cry1Ac gene.PDF