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Modified Organism
Bt okra
Record information and status
Record ID
115903
Status
Published
Date of creation
2021-02-19 17:57 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-02-19 17:57 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Bt okra
Transformation event
OE-17A
Developer(s)
Maharashtra Hybrid Seeds Company Limited (Mahyco)
Head Office
Jalna - Aurangabad Road,
Post Box no. 76,
Dawalwadi, Jalna
Dawalwadi, Maharashtra
India, 431203
Phone:+91 02482-262471
Fax:+91 02482-26002
Email:info@mahyco.com
Url:Mahyco Private Limited
Description
The okra (Abelmoschus esculentus) was modified for resistance to Lepidoptera pests (e.g. spotted bollworm) through the expression of Bacillus thuringiensis Cry1Ac. The protein forms pores in the midgut lining of susceptible pests, leading to cell lysis and septicemia. Additionally, the okra contains an Escherichia coli neomycin phosphotransferase II cassette for kanamycin selection during transformation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Abelmoschus esculentus - Okra, Lady's finger
Characteristics of the transformation process
Vector
pC2300cry1Ac10518ve
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Cry1Ac
0.00 Kb
 
 
transcription stop
 
 
CaMV 35S promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The modified okra includes two gene cassettes: Bacillus thuringiensis cry1Ac and Escherichia coli neomycin phosphotransferase II (nptII).

The cry1Ac is under control of the Cauliflower mosaic virus (CaMV) 35S enhanced promoter and a poly(A) terminator. Due to the constitutive nature of the promoter, transcription is expected to occur at high levels.

Transcription of nptII is under control of the CaMV 35S promoter and 35S terminator. Again, transcription is expected to occur at high levels due to the nature of the viral promoter.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Food