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Modified Organism
Bt cauliflower
Record information and status
Record ID
115912
Status
Published
Date of creation
2021-02-23 21:08 UTC (austein.mcloughlin@cbd.int)
Date of last update
2021-02-23 21:51 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-02-23 21:51 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Bt cauliflower
Transformation event
CFE-4
Developer(s)
Maharashtra Hybrid Seeds Company Limited (Mahyco)
Head Office
Jalna - Aurangabad Road,
Post Box no. 76,
Dawalwadi, Jalna
Dawalwadi, Maharashtra
India, 431203
Phone:+91 02482-262471
Fax:+91 02482-26002
Email:info@mahyco.com
Url:Mahyco Private Limited
Description
The cauliflower (Brassica oleracea) was modified for resistance to Lepidoptera pests through the introduction and expression of Bacillus thuringiensis crystal protein, Cry1Ac. The protein forms pores in the midgut lining of susceptible pests, leading to cell lysis and septicemia. Additionally, the cauliflower contains an Escherichia coli neomycin phosphotransferase II cassette for kanamycin selection during transformation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica oleracea var. botrytis - Cauliflower, Broccoflower, Calabrese, Romanesci broccoli
Point of collection or acquisition of the recipient organism
Brassica oleracea Botrytis group
Characteristics of the transformation process
Vector
pC2300ve10518
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S terminator
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Neomycin Phosphotransferase II
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CaMV 35S promoter
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transcription stop
 
 
Cry1Ac
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CaMV Enhanced 35S promoter
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The modified cauliflower contains two gene cassettes: Escherichia coli neomycin phosphotransferase II (nptII) and Bacillus thuringiensis cry1Ac.

Transcription of nptII commences from a Cauliflower mosaic virus (CaMV) 35S promoter and terminates at a CaMV 35S terminator. Due to the viral promoters, transcription of nptII is expected to occur at elevated levels in all plant tissues.

Transcription of cry1Ac is controlled by a CaMV enhanced 35S promoter. Transcription is expected to occur at very high levels in all tissues due to the enhanced nature (duplicated enhancer) of the viral promoter.
LMO characteristics
Modified traits
Common use(s)
  • Food
Detection method(s)
Additional information
See provision patent attached in the 'Additional Information' section below.