Record details

Living Modified Organism identity

The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name

Bt cauliflower

Transformation event

CFE-4

Developer(s)

Record #115887

Maharashtra Hybrid Seeds Company Limited (Mahyco)

Head Office

Jalna - Aurangabad Road,
Post Box no. 76,
Dawalwadi, Jalna

Dawalwadi, Maharashtra

India, 431203

Phone:	+91 02482-262471
Fax:	+91 02482-26002
Email:	info@mahyco.com
Url:	Mahyco Private Limited

Description

The cauliflower (Brassica oleracea) was modified for resistance to Lepidoptera pests through the introduction and expression of Bacillus thuringiensis crystal protein, Cry1Ac. The protein forms pores in the midgut lining of susceptible pests, leading to cell lysis and septicemia. Additionally, the cauliflower contains an Escherichia coli neomycin phosphotransferase II cassette for kanamycin selection during transformation.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #115913

Brassica oleracea var. botrytis - Cauliflower, Broccoflower, Calabrese, Romanesci broccoli

Point of collection or acquisition of the recipient organism

Brassica oleracea Botrytis group

Characteristics of the transformation process

Vector

pC2300ve10518

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

CaMV 35S terminator

#100290

0.00 Kb

Neomycin Phosphotransferase II

#15001

0.00 Kb

CaMV 35S promoter

#100287

0.00 Kb

transcription stop

(antisense)

Cry1Ac

#14986

0.00 Kb

CaMV Enhanced 35S promoter

#100366

0.00 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

The modified cauliflower contains two gene cassettes: Escherichia coli neomycin phosphotransferase II (nptII) and Bacillus thuringiensis cry1Ac.

Transcription of nptII commences from a Cauliflower mosaic virus (CaMV) 35S promoter and terminates at a CaMV 35S terminator. Due to the viral promoters, transcription of nptII is expected to occur at elevated levels in all plant tissues.

Transcription of cry1Ac is controlled by a CaMV enhanced 35S promoter. Transcription is expected to occur at very high levels in all tissues due to the enhanced nature (duplicated enhancer) of the viral promoter.

LMO characteristics

Modified traits

Resistance to antibiotics
- Kanamycin
Resistance to diseases and pests
- Insects
  - Lepidoptera (butterflies and moths)

Common use(s)

Food

Detection method(s)

Additional information

See provision patent attached in the 'Additional Information' section below.

Additional Information

Other relevant website address or attached documents

Insect resistant transgenic cauliflower plant comprising CFE-4 and methods of detection thereof.pdf

Bt Cauliflower construct.pdf