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Modified Organism
Bt rice
Record information and status
Record ID
115914
Status
Published
Date of creation
2021-02-24 20:35 UTC (austein.mcloughlin@cbd.int)
Date of last update
2021-02-24 20:45 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-02-24 20:45 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Bt rice
Transformation event
PE-2
Developer(s)
Maharashtra Hybrid Seeds Company Limited (Mahyco)
Head Office
Jalna - Aurangabad Road,
Post Box no. 76,
Dawalwadi, Jalna
Dawalwadi, Maharashtra
India, 431203
Phone:+91 02482-262471
Fax:+91 02482-26002
Email:info@mahyco.com
Url:Mahyco Private Limited
Description
The rice (Oryza sativa) was modified for resistance to Lepidoptera pests (e.g. yellow stem borer) through the expression of Bacillus thuringiensis Cry1Ac. The protein forms pores in the midgut lining of susceptible pests, leading to cell lysis and septicemia. Additionally, the rice contains an Escherichia coli hygromycin B phosphotransferase cassette for hygromycin selection during transformation and beta-glucuronidase as a reporter.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Oryza sativa - Rice, ORYSA
Point of collection or acquisition of the recipient organism
Rice line MPH-I
Related LMOs
Bt rice
Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
Bt rice
Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
Characteristics of the transformation process
Vector
pMH0102 and pCAMBIA1201
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Cry1Ac
0.00 Kb
 
 
transcription stop
 
 
CaMV 35S terminator
0.17 Kb
 
 
Hygromycin B phosphotransferase gene
1.03 Kb
 
 
CaMV Enhanced 35S promoter
0.68 Kb
 
 
CaMV 35S promoter
0.35 Kb
 
 
Catalase 1 intron
0.19 Kb
 
 
Beta-glucuronidase coding sequence
1.83 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The rice was modified using Agrobacterium-mediated transformation. The Rhizobium radiobacter (Agrobacterium) contained two transformation plasmids pMH0102 (Bacillus thuringiensis crystal protein cry1Ab) and pCAMBIA1201 (Escherichia coli hygromycin B phosphotransferase (hpt) and E. coli beta-glucuronidase (gus)).

Transcription of  cry1Ac starts from the Cauliflower mosaic virus (CaMV) enhanced 35S promoter and ends at a terminator. Transcription is expected to occur at elevated levels due to the nature of the CaMV promoter.

Transcription of hpt is under control of a CaMV enhanced 35S promoter and CaMV 35S terminator. Due to the nature of the CaMV promoter, transcription is expected to occur at elevated levels.

Transcription of gus is under control of a CaMV 35S promoter and a R. radiobacter nopaline synthase terminator. The gus sequence has a Ricinus communis catalase 1 intron at the 5' of the coding sequence for expression in plant, but not bacterial systems. The CaMV promoter induces high levels of transcription.

Note:
- Segregation analysis indicated that the genome contains a single insertion of the cry1Ac coding sequence.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Feed
Detection method(s)
Additional information
Kindly refer to the attached provisional patent in the 'Additional information' section below.

Records referencing this document (2)
IDDescription
2record(s) found
Modified Organism2 records

   
   
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