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Information Resource
Record information and status
Record ID
115947
Status
Published
Date of creation
2021-03-23 20:24 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-03-23 20:24 UTC (austein.mcloughlin@cbd.int)

General Information
Title
Optimization of Loop-Mediated Isothermal Amplification (LAMP) reaction mixture for biosensor applications
Author
Sümeyra Vural Kaymaz, Meltem Elitas
Author’s contact information
Meltem Elitas
Faculty of Engineering and Natural Sciences, Sabanci University
Istanbul 34956, Turkey

Kindly refer to the attached article for the corresponding author's contact information
Language(s)
  • English
Publication date
2021-02-19
Subject
Summary, abstract or table of contents
Genetically Modified (GM) foods are becoming the future of agriculture on surviving global natural disasters and climate change by their enhanced production efficiency and improved functional properties. On the other hand, their adverse health and environmental effects, ample evidence on transgene leakage of Genetically Modified Organisms (GMOs) to crops have raised questions on their benefits and risks. Consequently, low-cost, reliable, rapid, and practical detection of GMOs have been important. GMO-detection platforms should be capable of stably storing detection reagents for long-delivery distances with varying ambient temperatures. In this study, we developed an event-specific, closed tube colorimetric GMO detection method based on Loop-Mediated Isothermal Amplification (LAMP) technique which can be integrated into GMO-detection platforms. The entire detection process optimized to 30 min and isothermally at 65°C. The durability of the LAMP mixture in the test tubes showed that the LAMP reaction mixture, in which Bst polymerase and DNA sample was later included, yielded DNA amplicons for 3 days at room temperature, and for 6 days at 4°C.
• Simple, stable, and cheap storage method of LAMP reaction mixture for GMO-detection technologies.
• GMO-detection platforms can stably store detection reagents for long-delivery distances with varying ambient temperatures.
• Any DNA sample can be used in the field or resource-limited setting by untrained personnel.
Thematic areas
Information on Organisms or LMOs
Organism(s) identification
Glycine max - Soybean, Soya bean, Soya, SOYBN
Gene(s) identification
CaMV 35S promoter - Cauliflower mosaic virus - CaMV
LMO(s) identification
MON-Ø4Ø32-6 - Roundup Ready™ soybean
Resistance to herbicides - Glyphosate
Show detection method(s)
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
Identifier
doi.org/10.1016/j.mex.2021.101282
Publisher and its location
MethodsX
Elsevier
Format
PDF - 748 KB (8 pages)
Keywords and any other relevant information
Mediated Isothermal Amplification (LAMP); foods; LMO; Resource-limited settings; Storage; In field testing