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Modified Organism
ACS-BVØØ1-3 - Liberty Link™ sugarbeet
Record information and status
Record ID
14763
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-05-15 14:00 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-05-15 14:00 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Liberty Link™ sugarbeet
Transformation event
T120-7
Unique identifier
ACS-BVØØ1-3
Developer(s)
Description
Sugar beet tolerant to the herbicide glufosinate, created through introduction of the phosphinothricin acetyltransferase (pat) gene from Streptomyces viridochromogenes, an aerobic soil bacteria, which confers tolerance to the herbicide Phosphinothricin (Glufosinate ammonium).  Neomycin phosphotransferase II (npt II) confers tolerance to the antibiotic kanamycin.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Beta vulgaris - Common beet, Sugarbeet, BETMA
Characteristics of the transformation process
Vector
pOCA18/Ac
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.53 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.23 Kb
 
 
Nopaline Synthase Gene Promoter
0.34 Kb
 
 
Neomycin Phosphotransferase II
0.80 Kb
 
 
Octopine Synthase Gene Terminator
0.79 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The native pat gene was modified to reduce the G/C content of the DNA sequence and to a plant preferred codon sequence. The native and modified genes share 70% sequence homology however there was no change to the amino acid sequence.

Southern blot and PCR analysis indicated that a single insert of the T-DNA was integrated into the host genome and single copies of each of the bar and nptII genes.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
The sugar beet line T120-7 was genetically engineered to express tolerance to glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta®, Rely®, Finale®, and Liberty®). Glufosinate ammonium acts by inhibiting the plant enzyme glutamine synthetase, a key enzyme that detoxifies ammonia by incorporating it into glutamine. Inhibition of this enzyme leads to an accumulation of ammonia in the plant tissues, which kills the plant within hours of application. Phosphinothricin herbicides applied at rates recommended for effective weed control are toxic to conventional sugar beet varieties. The modified T120-7 line permits growers to use phosphinothricin-containing herbicides for weed control in the cultivation of sugar beet.

Glufosinate tolerance in T120-7 sugar beet is the result of the introduction of the pat gene into the beet genome via Agrobacterium-mediated transformation. The pat gene was isolated from the common soil fungus, Streptomyces viridochromogenes, and encodes the enzyme phosphinothricin-N-acetyltransferase (PAT). PAT catalyses the acetylation of phosphinothricin which detoxifies it into an inactive compound.
Other relevant website address or attached documents

Records referencing this document (7)
IDDescription
7record(s) found
Country's Decision or any other Communication3 records
Organization1 record
Risk Assessment3 records