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Modified Organism
DD-Ø26ØØ5-3 - High oleic acid soybean
Record information and status
Record ID
14769
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2014-01-16 21:17 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-01-16 21:17 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
High oleic acid soybean
Transformation event
260-05 (Lines: G94-1, G94-19, G-168)
Unique identifier
DD-Ø26ØØ5-3
Developer(s)
Pioneer Hi-Bred International Inc.
Url:Pioneer HiBred International Homepage
Description
Soybean lines G94-1, G94-19 and G-168 were developed through a specific genetic modification to produce a soybean oil that contains high levels of oleic acid, a monounsaturated fatty acid. These high oleic soybeans contain a second copy of fatty acid desaturase gene (fad2), which is naturally present in soybeans. The fad2 gene codes for the enzyme, delta-12 desaturase, which is involved in fatty acid synthesis. Unlike conventional soybeans, the presence of a second copy of the fad2 gene in the high oleic soybeans G94-1, G94-19 and G-168 causes a phenomenon known as "sense suppression" which results in both copies of the fatty acid desaturase gene being "switched off". This blocks the fatty acid biosynthetic pathway and results in the accumulation of oleic acid. As a consequence, polyunsaturated fatty acids (linoleic acid and linolenic acid) are only produced in very small amounts.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Characteristics of the transformation process
Vector
pBS43 and pML102
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
α' subunit of β-conglycinin gene promoter
0.61 Kb
 
 
delta(12)-fatty acid dehydrogenase
1.46 Kb
 
 
Phaseolin 3' Terminator
1.17 Kb
 
 
CaMV 35S promoter
1.40 Kb
 
 
5' untranslated leader of chlorophyll a/b-binding protein
0.06 Kb
 
 
Beta-Glucuronidase coding sequence
1.85 Kb
 
 
Nopaline Synthase Gene Terminator
0.77 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
High oleic acid soybean produced by inserting a second copy of the fatty acid desaturase (GmFad2-1) gene from soybean, which resulted in "silencing" of the endogenous host gene.

The transgenic fad2 gene in G94-1, G94-19 and G-168 was isolated from Glycine max (soybean) and introduced into a commercial soybean variety using particle acceleration (biolistic) transformation. The genetic modification affects only the seed, allowing fatty acid biosynthesis to function normally in other plant parts such as the leaves.

The original transformant was co-bombarded with vector pML102 which contained the dapA gene from the bacterium Corynebacterium glutanicum, encoding dihydrodipicolinic acid synthase, expression of which can lead to an increase in free lysine content. The dapA gene was inserted independently from the GmFad2-1 gene and was lost via segregation, and is thus not present in the subject soybean lines.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
High oleic soybean oil contains levels of oleic acid exceeding 80%, higher than the levels found in olive oil and rapeseed oil. This oil is lower in saturated fat, contains no trans-fatty acids, and remains in a liquid form. The high levels of oleic acid make the oil more heat-stable for cooking and edible spray applications.
Other relevant website address or attached documents

Records referencing this document (6)
IDDescription
6record(s) found
Country's Decision or any other Communication4 records
Risk Assessment2 records