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Modified Organism
FLO-11363-2 - Moonshadow™ carnation
Record information and status
Record ID
14772
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2015-01-26 21:47 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-01-26 21:47 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Moonshadow™ carnation
Transformation event
11363 (1363A)
Unique identifier
FLO-11363-2
Developer(s)
Stephen Chandler
Cosnultant
SUntory Holdings Ltd.
Melbourne, VIC
Australia
Phone:+61 409 387 386
Email:schandler@florigene.com.au
Url:Florigene Homepage
Description
Carnation Moonshadow 1363A has a modified flower colour, a shade of light mauve, whereas the non-GM parent has cream-white flowers. The colour has been achieved by introducing into white carnation two genes of the anthocyanin biosynthesis pathway from Petunia and Viola sp. These genes, encoding dihydroflavonol 4-reductase (dfr) and flavonoid 3'5' hydroxylase (f3'5'h), together with other genes of the anthocyanin biosynthesis pathway already present in the non GM carnation, give rise to the anthocyanins delphinidin and cyanidin.

Note: No longer available, was sold for about 10 years, has been replaced by superior varieties

NOTE: This LMO was formerly referred to with the UID FLO-11363-1.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Dianthus caryophyllus - Carnation, DIACA
Point of collection or acquisition of the recipient organism
Cultivar: White Unesco
Related LMOs
FLO-4Ø689-6 - Moonaqua™ carnation
Dr Yoshikazu Tanaka Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-11226-9 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-4Ø685-2 - Moonvista™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-11351-8 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-114ØØ-3 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-11959-4 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-11988-6 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
Characteristics of the transformation process
Vector
pCGP1991
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.20 Kb
 
 
5' untranslated leader of chlorophyll a/b-binding protein
0.06 Kb
 
 
Acetohydroxy acid synthase gene
3.77 Kb
 
 
Acetohydroxy acid synthase gene terminator
0.00 Kb
 
 
Dihydroflavonol-4-reductase promoter
0.00 Kb
 
 
Dihydroflavonol-4-reductase
4.96 Kb
 
 
Dihydroflavonol-4-reductase terminator
0.00 Kb
 
 
Chalcone synthase gene promoter
1.17 Kb
 
 
Flavonoid 3’, 5’-hydroxylase gene
1.80 Kb
 
 
D8 gene terminator
0.81 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Southern blot analysis indicated that there are 4 to 6 copies of the insert present in FLO-11363-1. Three integration sites were sequenced, each of which consist of both complete and partial T-DNA sequences. Locus 1 consists of three parts of the T-DNA of which two parts are in a sense orientation and one in anti-sense orientation. Locus 2 consists of a partial T-DNA in the anti-sense orientation and one complete T-DNA in the sense orientation. Locus 3 consists of two complete T-DNA in the sense orientation and a partial T-DNA in the anti-sense orientation. 

Southern blot analysis also indicated the absence of vector backbone sequences.
LMO characteristics
Modified traits
Common use(s)
  • Ornamental
Additional Information
Additional Information
Three genes have been transferred into FLO-4Ø689-6, these are:

• The petunia DFR gene, coding for dihydroflavonol 4-reductase (DFR), derived from Petunia X hybrida. The petunia DFR enzyme is only capable of using dihydroquercetin and dihydromyricetin as substrate, not dihydrokaempferol. This ensures that most or all of the anthocyanidin produced is delphinidin. A constitutive promoter drives the petunia DFR-A cDNA derived gene;

• the pansy F3'5'H gene, coding for flavonoid 3' 5' hydroxylase (F3'5'H), derived from Viola sp. F3'5'H acts by converting the dihydroflavonols dihydrokaempferol and/or dihydroquercetin into the dihydroflavonol dihydromyricetin. The cDNA for F3'5'H encodes the enzyme F3'5'H allowing transgenic plants normally lacking this enzyme to produce violet or blue delphinidin derived pigments; and

• ALS gene (SuRB), coding for a mutant acetolactate synthase protein (ALS), derived from Nicotiana tabacum. Expression of the mutation confers resistance to sulfonylurea herbicides.
Other relevant website address or attached documents

Records referencing this document (14)
IDDescription
14record(s) found
Country's Decision or any other Communication3 records
Modified Organism8 records
Risk Assessment3 records