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Modified Organism
CGN-89322-3 - Delayed-ripening tomato
Record information and status
Record ID
14781
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2014-01-17 20:32 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-01-17 20:32 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Delayed-ripening tomato
Transformation event
8338
Unique identifier
CGN-89322-3
Developer(s)
Monsanto (Calgene)
Url:Monsanto Homepage
Description
Tomato line 8338 was developed by introducing into the genome of a processing tomato cultivar UC82B a gene for 1-amino-cyclopropane-1-carboxylic acid deaminase (ACCd) derived from a nonpathogenic soil bacterium (Pseudomonas chlororaphis). In the plant, this enzyme catalyzes metabolism of 1-amino-cyclopropane-1-arboxylic acid (ACC), an essential precursor for the biosynthesis of the plant ripening hormone ethylene.

The initiation and progression of tomato fruit ripening depends on increased levels of ethylene. In line 8338, ACC is sufficiently reduced in detached fruit so that ethylene becomes limiting and the ripening process is delayed. Line 8338 has also been transformed with the nptII gene derived from Escherichia coli that encodes the enzyme NPTII. NPTII confers resistance to certain antibiotics, such as kanamycin, that are used to select transformed cells.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum lycopersicum - Tomato, SOLLC
Point of collection or acquisition of the recipient organism
Cultivar: UC82B
Characteristics of the transformation process
Vector
PV-LERP07
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
FMV 35S promoter
0.57 Kb
 
 
HSP 70 5' untranslated leader sequence
0.11 Kb
 
 
1-amino-cyclopropane-1-carboxylic acid deaminase
1.08 Kb
 
 
rbcS-E9 gene terminator
0.66 Kb
 
 
CaMV 35S promoter
0.37 Kb
 
 
Neomycin Phosphotransferase II
0.85 Kb
 
 
Octopine Synthase Gene Terminator
0.27 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
1-amino-cyclopropane-1-carboxylic acid deaminase - Pseudomonas chlororaphis strain 6G5
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Other relevant website address or attached documents

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