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Modified Organism
PH-ØØØ676-7 - Male-sterile, herbicide-tolerant maize
Record information and status
Record ID
14791
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2012-09-14 14:11 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-09-14 14:11 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Male-sterile, herbicide-tolerant maize
Transformation event
676
Unique identifier
PH-ØØØ676-7
Developer(s)
Pioneer Hi-Bred International Inc.
Url:Pioneer HiBred International Homepage
Description
The maize lines 676, 678, and 680 were genetically engineered to express male sterility and tolerance to glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta®, Rely®, Finale®, and Liberty®).

The male-sterile trait was introduced by inserting a bacterial gene encoding the enzyme DNA adenine methylase (DAM). Expression of the Escherichia coli dam gene in specific plant tissues results in the inability of the transformed plants to produce anthers or pollen, resulting in a male-sterile plant. The PAT enzyme was used as a selectable marker enabling identification of transformed plants during tissue culture regeneration, and as a field selection method to identify the male-sterile lines prior to flowering. Under field conditions, plants that were not male-sterile could be eliminated by application of the herbicide glufosinate ammonium. The novel hybrid system provided an efficient and effective way to identify male-sterile plants for use in hybrid seed production.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Related LMOs
PH-ØØØ678-9 - Male-sterile, herbicide-tolerant maize
Changes in physiology and/or production - Reproduction - Male sterility Resistance to herbicides - Glufosinate
PH-ØØØ68Ø-2 - Male-sterile, herbicide-tolerant maize
Changes in physiology and/or production - Reproduction - Male sterility Resistance to herbicides - Glufosinate
Characteristics of the transformation process
Vector
PHP6710
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
5126del Anther Specific Promoter
0.00 Kb
 
 
DNA Adenine Methylase gene
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Proteinase inhibitor II gene terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Both genes are inserted as a single copy locus in the genome. A partial copy of the pat gene insertion was also detected at another locus in the genome.
LMO characteristics
Modified traits
Common use(s)
  • Feed
Additional Information
Additional Information
Glufosinate tolerance in these maize lines is the result of introducing a gene encoding the enzyme phosphinothricin-N-acetyltransferase (PAT) isolated from the common aerobic soil actinomycete, Streptomyces viridochromogenes, the same organism from which glufosinate was originally isolated. The PAT enzyme catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound. The PAT enzyme is not known to have any toxic properties.

Glufosinate chemically resembles the amino acid glutamate and acts to inhibit an enzyme, called glutamine synthetase, which is involved in the synthesis of glutamine. Essentially, glufosinate acts enough like glutamate, the molecule used by glutamine synthetase to make glutamine, that it blocks the enzyme's usual activity. Glutamine synthetase is also involved in ammonia detoxification. The action of glufosinate results in reduced glutamine levels and a corresponding increase in concentrations of ammonia in plant tissues, leading to cell membrane disruption and cessation of photosynthesis resulting in plant withering and death.
Other relevant website address or attached documents

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication1 record
Modified Organism2 records
Risk Assessment1 record