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The soybean line GTS 40-3-2 was developed to allow for the use of
glyphosate, the active ingredient in the herbicide Roundup®, as a
weed control option. This genetically engineered soybean line
contains a form of the plant enzyme
5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows GTS
40-3-2 to survive the otherwise lethal application of glyphosate.
The EPSPS gene put into GTS 40-3-2 was isolated from a strain of
the common soil bacterium Agrobacterium tumefaciens called CP4 and
the form of EPSPS enzyme produced by this gene is tolerant to
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
CaMV Enhanced 35S promoter
Chloroplast Transit Peptide 4
Nopaline Synthase Gene Terminator
The plasmid PV-GMGT04 contained three transformation cassettes
driven by plant promoters: two cp4 epsps genes and a gene encoding
ß-glucuronidase (GUS) from E. coli. Only a portion of this
vector was incorporated into event 40-3-2. Southern blot and PCR
analysis indicated that only a single transformation cassette
containing the EPSPS coding sequence was integrated into the host
genome. Analysis also indicated that there was no intigration of
segments of the vector backbone, the GUS coding sequence or the
The cp4epsps coding sequence encodes a 455 amino acid protein is
terminated by tandem stop codons, and results in the synthesis of
the full length and functional ~46kDa CP4EPSPS protein in
Round-up Ready soybean event 40-3-2 as confirmed by western
blotting, enzyme-linked immunosorbent assay and EPSPS enzyme
The EPSPS enzyme is part of an important biochemical pathway in
plants called the shikimate pathway, that is involved in the
production of aromatic amino acids and other aromatic compounds.
When conventional plants are treated with glyphosate, the plants
cannot produce the aromatic amino acids needed to grow and survive.
EPSPS is present in all plants, bacteria, and fungi. It is not
present in animals, which do not synthesize their own aromatic
amino acids. Because the aromatic amino acid biosynthetic pathway
is not present in mammals, birds or aquatic life forms, glyphosate
has little if any toxicity for these organisms. The EPSPS enzyme is
naturally present in foods derived from plant and microbial