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Modified Organism
MON-Ø4Ø32-6 - Roundup Ready™ soybean
Record information and status
Record ID
14796
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2014-01-21 20:12 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-01-21 20:12 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Roundup Ready™ soybean
Transformation event
GTS 40-3-2 (40-3-2)
Unique identifier
MON-Ø4Ø32-6
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
The soybean line GTS 40-3-2 was developed to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. This genetically engineered soybean line contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows GTS 40-3-2 to survive the otherwise lethal application of glyphosate. The EPSPS gene put into GTS 40-3-2 was isolated from a strain of the common soil bacterium Agrobacterium tumefaciens called CP4 and the form of EPSPS enzyme produced by this gene is tolerant to glyphosate.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Point of collection or acquisition of the recipient organism
Line: A5403
Characteristics of the transformation process
Vector
PV-GMGT04
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
Chloroplast Transit Peptide 4
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.36 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The plasmid PV-GMGT04 contained three transformation cassettes driven by plant promoters: two cp4 epsps genes and a gene encoding ß-glucuronidase (GUS) from E. coli. Only a portion of  this vector was incorporated into event 40-3-2. Southern blot and PCR analysis indicated that only a single transformation cassette containing the EPSPS coding sequence was integrated into the host genome. The largest insert, containing the function CP4 EPSPS gene, contained a deletion in the enhancer region of the E35S promoter and the remainder of the E35S promoter was functional. Analysis also indicated that there was no intigration of segments of the vector backbone, the GUS coding sequence or the CmoVb promoter.

The cp4epsps coding sequence encodes a 455 amino acid protein is terminated by tandem stop codons, and results in the synthesis of the full length and functional  ~46kDa CP4EPSPS protein in Round-up Ready soybean event 40-3-2 as confirmed by western blotting, enzyme-linked immunosorbent assay and EPSPS enzyme activity assays.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
The EPSPS enzyme is part of an important biochemical pathway in plants called the shikimate pathway, that is involved in the production of aromatic amino acids and other aromatic compounds. When conventional plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to grow and survive. EPSPS is present in all plants, bacteria, and fungi. It is not present in animals, which do not synthesize their own aromatic amino acids. Because the aromatic amino acid biosynthetic pathway is not present in mammals, birds or aquatic life forms, glyphosate has little if any toxicity for these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources.
Other relevant website address or attached documents

Records referencing this document (109)
IDDescription
109record(s) found
Country's Decision or any other Communication41 records
Information Resource5 records
Modified Organism3 records
Organization29 records
Risk Assessment31 records