Record details

Living Modified Organism identity

The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name

Roundup Ready™ soybean

Transformation event

GTS 40-3-2 (40-3-2)

Unique identifier

MON-Ø4Ø32-6

Developer(s)

Monsanto

Url:	Monsanto Homepage

Record #14925

Description

The soybean line GTS 40-3-2 was developed to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. This genetically engineered soybean line contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows GTS 40-3-2 to survive the otherwise lethal application of glyphosate. The EPSPS gene put into GTS 40-3-2 was isolated from a strain of the common soil bacterium Agrobacterium tumefaciens called CP4 and the form of EPSPS enzyme produced by this gene is tolerant to glyphosate.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Glycine max - Soybean, Soya bean, Soya

Record #10453

Point of collection or acquisition of the recipient organism

Line: A5403

Characteristics of the transformation process

Vector

PV-GMGT04

Techniques used for the modification

Biolistic / Particle gun

Genetic elements construct

CaMV Enhanced 35S promoter

#100366

0.61 Kb

Chloroplast Transit Peptide 4

#103899

0.23 Kb

5-enolpyruvylshikimate-3-phosphate synthase

#14979

1.36 Kb

Nopaline Synthase Gene Terminator

#100269

0.26 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

The plasmid PV-GMGT04 contained three transformation cassettes driven by plant promoters: two cp4 epsps genes and a gene encoding ß-glucuronidase (GUS) from E. coli. Only a portion of this vector was incorporated into event 40-3-2. Southern blot and PCR analysis indicated that only a single transformation cassette containing the EPSPS coding sequence was integrated into the host genome. Analysis also indicated that there was no intigration of segments of the vector backbone, the GUS coding sequence or the CmoVb promoter.

The cp4epsps coding sequence encodes a 455 amino acid protein is terminated by tandem stop codons, and results in the synthesis of the full length and functional ~46kDa CP4EPSPS protein in Round-up Ready soybean event 40-3-2 as confirmed by western blotting, enzyme-linked immunosorbent assay and EPSPS enzyme activity assays.

LMO characteristics

Modified traits

Resistance to herbicides
- Glyphosate

Common use(s)

Food
Feed

Detection method(s)

External link(s)

MON-Ø4Ø32-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

MON-Ø4Ø32-6 - CropLife International Detection Methods Database

Additional Information

The EPSPS enzyme is part of an important biochemical pathway in plants called the shikimate pathway, that is involved in the production of aromatic amino acids and other aromatic compounds. When conventional plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to grow and survive. EPSPS is present in all plants, bacteria, and fungi. It is not present in animals, which do not synthesize their own aromatic amino acids. Because the aromatic amino acid biosynthetic pathway is not present in mammals, birds or aquatic life forms, glyphosate has little if any toxicity for these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources.

Other relevant website address or attached documents

CERA GM Database

MON-Ø4Ø32-6 - Monsanto.pdf

MON-Ø4Ø32-6 - OECD