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Modified Organism
DAS-Ø15Ø7-1 x MON-ØØ6Ø3-6 - Herculex™ I Roundup Ready™ 2 maize
Record information and status
Record ID
14808
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-07-23 20:30 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-23 20:30 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herculex™ I Roundup Ready™ 2 maize
Transformation event
TC1507 x NK603
Unique identifier
DAS-Ø15Ø7-1 x MON-ØØ6Ø3-6
Developer(s)
Pioneer Hi-Bred International Inc.
Url:Pioneer HiBred International Homepage
Description
This LMO is the result of a cross between LMOs DAS-Ø15Ø7-1 and MON-ØØ6Ø3-6.

The MON-ØØ6Ø3-6 line of maize was developed to allow the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. NK603 contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate.

Corn line DAS-Ø15Ø7-1 is modified with the insertion of the Cry1F gene from Bacillus thuringiensis to confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
DAS-Ø15Ø7-1 - Herculex™ I maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
MON-ØØ6Ø3-6 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
PHP8999 and PV-ZMGT32
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Ubiquitin gene promoter
1.98 Kb
 
 
Cry1F
1.82 Kb
 
 
ORF25 PolyA Terminator sequence
0.72 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
 
Rice actin 1 gene promoter
0.80 Kb
 
 
Rice actin 1, intron
0.60 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
 
CaMV Enhanced 35S promoter
0.60 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Notes about the Other(s) sequence(s) specific to this LMO

DNA construct from TC1507:

P-ubiZM1 (1.98) >> cry1F (1.82) >> T-ORF25 (0.72)* | P-35S (0.55) >> pat (0.55) >> T-35s (0.2)

DNA construct from NK603:

P-Ract1 >> I-Ract1 >> TS-CTP2 >> cp4-epsps >> T-nos | spacer (217) |  P-e35S >> I-Hsp70  >> TS-CTP2 >> cp4-epsps >> T-nos

Note: the symbols >> and << indicate the sense and anti-sense direction of transcription, respectively, between different elements (typically, promoter - coding sequence - terminator). If present, the symbol | indicates elements that may be cloned adjacently in a cassette but that presumably are not transcribed together. The numbers in parenthesis indicate the approximate length in Kb of each genetic element.

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* The EFSA document attached below indicates that the 3' sequence from the Agrobacterium tumefaciens mannopine synthase gene was used as terminator of the cry1F gene.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel

Records referencing this document (101)
IDDescription
101record(s) found
Country's Decision or any other Communication45 records
Information Resource2 records
Modified Organism1 record
Organization7 records
Risk Assessment46 records