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Modified Organism
ACS-GMØØ2-9 - Herbicide-tolerant soybean
Record information and status
Record ID
14853
Status
Published
Date of creation
2006-06-05 14:40 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-01-18 19:38 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-01-18 19:38 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide-tolerant soybean
Transformation event
W62
Unique identifier
ACS-GMØØ2-9
Developer(s)
Description
The soybean lines W62 and W98 were genetically engineered to express tolerance to glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta®, Rely®, Finale®, and Liberty®). Glufosinate chemically resembles the amino acid glutamate and acts to inhibit an enzyme, called glutamine synthetase, which is involved in the synthesis of glutamine. Essentially, glufosinate acts enough like glutamate, the molecule used by glutamine synthetase to make glutamine, that it blocks the enzyme's usual activity. Glutamine synthetase is also involved in ammonia detoxification. The action of glufosinate results in reduced glutamine levels and a corresponding increase in concentrations of ammonia in plant tissues, leading to cell membrane disruption and cessation of photosynthesis resulting in plant withering and death.

Glufosinate tolerance in W62 and W98 soybean lines is the result of introducing a gene encoding the enzyme phosphinothricin-N-acetyltransferase (PAT) isolated from the common aerobic soil actinomycete, Streptomyces hygroscopicus, the same organism from which glufosinate was originally isolated. The PAT enzyme catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound. The PAT enzyme is not known to have any toxic properties. The PAT encoding gene (bar) was introduced into the soybean genome by micro-particle acceleration (biolistic) transformation, and the resulting soybean lines displayed field tolerance to phosphinothricin-containing herbicides, thereby permitting farmers to use this herbicide for weed control in soybean cultivation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Related LMOs
ACS-GMØØ5-3 - Herbicide-tolerant soybean
Resistance to herbicides - Glufosinate
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ACS-GMØØ1-8 - Herbicide-tolerant soybean
Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate Selectable marker genes and reporter genes
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ACS-GMØØ3-1 - Herbicide-tolerant soybean
Resistance to herbicides - Glufosinate
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ACS-GMØØ4-2 - Herbicide-tolerant soybean
Resistance to herbicides - Glufosinate
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ACS-GMØØ6-4 - Liberty Link™ soybean
Resistance to herbicides - Glufosinate
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ACS-GMØØ8-6 - Herbicide-tolerant soybean
Resistance to herbicides - Glufosinate
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Characteristics of the transformation process
Vector
pWRG2114 (AKA: CMC2114)
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
CaMV 35S promoter
0.43 Kb
 
 
5' Untranslated Leader of AMV RNA4
0.04 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
RuBisCo SSU Terminator
0.32 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
Beta-Glucuronidase coding sequence
1.81 Kb
 
 
5' Untranslated Leader of AMV RNA4
0.04 Kb
 
 
CaMV 35S promoter
0.43 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The ampicillan resistance gene, beta-lactamase, was also integrated into the host genome.
LMO characteristics
Modified traits
Common use(s)
  • Food
Detection method(s)
Additional information
Southern Blot analysis indicated that the W98 line contains approximately 2 intact copies of the BAR, GUS and ampr genes.
Additional Information
Other relevant website address or attached documents

Records referencing this document (7)
IDDescription
7record(s) found
Country's Decision or any other Communication1 record
Modified Organism6 records