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Modified Organism
ACS-GMØØ6-4 - Liberty Link™ soybean
Record information and status
Record ID
14857
Status
Published
Date of creation
2006-06-05 14:40 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-01-21 16:55 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-01-21 16:55 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Liberty Link™ soybean
Transformation event
A5547-127
Unique identifier
ACS-GMØØ6-4
Developer(s)
Description
The soybean line A5547-127 was developed to allow for the use of glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta®, Ignite®, Rely®, Liberty®, Harvest®, and Finale®) as a weed control option. This genetically engineered soybean line contains the fungal enzyme phosphinothricin-N-acetyltransferase (PAT), which allows these plants to survive the otherwise lethal application of glufosinate. The pat gene inserted into A5547-127 was isolated from a common soil fungus, Streptomyces viridochromogenes, and introduced into the soybean genome by particle acceleration (biolistic) transformation.

The PAT enzyme in soybean line A5547-127 converts L-phosphinothricin (PPT), the active ingredient in glufosinate ammonium, to an inactive form thereby conferring resistance to the herbicide. In the absence of PAT, application of glufosinate leads to reduced production of the amino acid glutamine and increased ammonia levels in the plant tissues, which are lethal to the plant. The PAT enzyme is not known to have any toxic properties.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Related LMOs
ACS-GMØØ5-3 - Herbicide-tolerant soybean
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ACS-GMØØ1-8 - Herbicide-tolerant soybean
Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate Selectable marker genes and reporter genes
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ACS-GMØØ2-9 - Herbicide-tolerant soybean
Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate Selectable marker genes and reporter genes
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ACS-GMØØ3-1 - Herbicide-tolerant soybean
Resistance to herbicides - Glufosinate
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ACS-GMØØ4-2 - Herbicide-tolerant soybean
Resistance to herbicides - Glufosinate
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ACS-GMØØ8-6 - Herbicide-tolerant soybean
Resistance to herbicides - Glufosinate
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Characteristics of the transformation process
Vector
pB2/35SAcK (AKA: pWRG5143)
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
CaMV 35S promoter
0.54 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The PAT gene from Streptomyces viridochromogenes was synthetically modified with plant preferred codons.

The bacterial ampicillin resistance gene was also partially integrated into the host genome. It is however not expressed in the host organism.
LMO characteristics
Modified traits
Common use(s)
  • Food
Detection method(s)
Additional information
Southern blot analysis indicated that 1 copy of the PAT gene integrated into the host genome.

One copy of the 5' portion of the bla gene was integrated upstream of the PAT gene and one copy of the 3' portion of the bla gene was integrated downstream of the PAT gene.
Additional Information
Other relevant website address or attached documents

Records referencing this document (51)
IDDescription
51record(s) found
Country's Decision or any other Communication20 records
Modified Organism8 records
Organization9 records
Risk Assessment14 records