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Modified Organism
ACS-OSØØ2-5 - Liberty Link™ rice
Record information and status
Record ID
14859
Status
Published
Date of creation
2006-06-05 14:40 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-05-06 19:43 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-05-06 19:43 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Liberty Link™ rice
Transformation event
LLRICE62
Unique identifier
ACS-OSØØ2-5
Developer(s)
Description
Rice resistant to the herbicide glufosinate through introduction of the bar gene from Streptomyces hygroscopicus which encodes the enzyme phosphinothricin-N-acetyltransferase (PAT) that catalyzes the acetylation of phosphinothricin (glufosinate), detoxifying it into an inactive compound.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Oryza sativa - Rice, ORYSA
Point of collection or acquisition of the recipient organism
Variety: Bengal
Related LMOs
ACS-OSØØ1-4 - Liberty Link™ rice
Resistance to herbicides - Glufosinate
BCS-OSØØ3-7 - Liberty Link™ rice
Resistance to herbicides - Glufosinate
Show detection method(s)
Characteristics of the transformation process
Vector
pB5/35Sbar
Techniques used for the modification
  • Direct DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.53 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.19 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The phosphinothricin-N-acetyltransferase enzyme coding sequence was obtained from the Streptomyces hygroscopicus HP632 strain. the The N-terminal codon of the wild type gene was modified to and ATG.

Southern blot analysis indicated that one intact copy of the transformation cassette integrated into the host genome. No portions of the vector backbone were detected in the LMO
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Additional Information
Rice line LLRICE62 was genetically engineered to express tolerance to glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta®, Rely®, Finale®, and Liberty®). Glufosinate chemically resembles the amino acid glutamate and acts to inhibit an enzyme, called glutamine synthetase, which is involved in the synthesis of glutamine. Essentially, glufosinate acts enough like glutamate, the molecule used by glutamine synthetase to make glutamine, that it blocks the enzyme's usual activity. Glutamine synthetase is also involved in ammonia detoxification. The action of glufosinate results in reduced glutamine levels and a corresponding increase in concentrations of ammonia in plant tissues, leading to cell membrane disruption and cessation of photosynthesis resulting in plant withering and death.

Glufosinate tolerance in these rice lines is the result of introducing a gene encoding the enzyme phosphinothricin-N-acetyltransferase (PAT) isolated from the common aerobic soil actinomycete, Streptomyces hygroscopicus, the same organism from which glufosinate was originally isolated. The PAT enzyme catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound. The PAT enzyme is not known to have any toxic properties. The PAT encoding gene (bar) was introduced into the rice genome by direct gene delivery transformation, and the resulting rice lines displayed field tolerance to phosphinothricin-containing herbicides.
Other relevant website address or attached documents

Records referencing this document (27)
IDDescription
27record(s) found
Country's Decision or any other Communication7 records
Modified Organism2 records
Organization13 records
Risk Assessment5 records