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Modified Organism
CGN-89564-2 - FLAVR SAVR™ tomato
Record information and status
Record ID
14867
Status
Published
Date of creation
2006-06-05 14:40 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2012-11-05 19:26 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-11-05 19:26 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
FLAVR SAVR™ tomato
Transformation event
Several lines
Unique identifier
CGN-89564-2
Developer(s)
Monsanto (Calgene)
Url:Monsanto Homepage
Description
The FLAVR SAVR™ tomato lines were genetically engineered to express delayed softening by insertion of a PG encoding gene in the "antisense" orientation, which causes the endogenous PG messenger RNA (mRNA) to be degraded and, consequently, less production of PG protein.

The measured level of endogenous PG activity in transgenic FLAVR SAVR tomato was found to be less than 1% of PG activity found in the unmodified recipient organism.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum lycopersicum - Tomato, SOLLC
Characteristics of the transformation process
Vector
Any of: pCGN1436, pCGN1547, pCGN1548, pCGN1549, pCGN1557, pCGN1558, pCGN1559, pCGN1578 or pCGN4109 depending on the line
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Polygalacturonase gene - Solanum lycopersicum - Tomato, SOLLC
Changes in physiology and/or production - Ripening
Neomycin Phosphotransferase II - Escherichia coli - ECOLX
Resistance to antibiotics - Kanamycin
Beta-galactosidase gene - Escherichia coli - ECOLX
Selectable marker genes and reporter genes
CaMV 35S promoter - Cauliflower mosaic virus - CaMV
Mannopine Synthase Gene Promoter - Arabidopsis thaliana - Thale cress, Mouse-ear cress, Arabidopsis, ARATH
Mannopine synthase gene terminator - Arabidopsis thaliana - Thale cress, Mouse-ear cress, Arabidopsis, ARATH
Ti plasmid left border repeat - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Ti plasmid right border repeat - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Tumour Morphology Large gene terminator - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Transcript 7 gene 3' untranslated region - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Notes regarding the genetic elements introduced or modified in this LMO
Notes about the Other(s) sequence(s) specific to this LMO

The pg gene from tomato was introduced in the anti-sense orientation to suppress the expression of the endogenous pg gene.

Depending on the construct/vector used (see below), the antisense pg gene was under regulatory control of a single or two tandem copies of the 35S promoter from the cauliflower mosaic virus (CaMV). The terminator sequences for the antisense pg gene were the tml gene (T-tml) and the transcript 7 gene from the octopine-type Ti plasmid pTiA6 from A. tumefaciens.

Expression of the nptII gene was under the control of the 5' promoter and 3' terminator sequences from the mannopine synthase gene derived from A. tumefaciens or the 35s promoter and T-tml terminator, depending on the construct introduced in each line.

The DNA cassettes in two of the vectors used are shown below. Information on the cassettes in other vectors used to modify FLAVR SAVR™ was not found.

DNA cassette in pCGN1436:
LB (0.58) | P-mas (0.68) >> nptII (0.98) >> T-mas (0.77) | lac (0.29) | P-35S (0.6) >> P-35S (0.6) << pg (1.6) << Space (0.012) >> T-tml (1.2) | lac (0.17) | RB (0.28)

DNA cassette in pCGN4109:
LB | T-tml << nptII << P-35S | T-tml >> pg >> P-35S (0.6) << P-35S (0.6) | RB

Note: the symbols >> and << indicate the sense and anti-sense direction of transcription, respectively, between different elements (typically, promoter - coding sequence - terminator). If present, the symbol | indicates elements that may be cloned adjacently in a cassette but that presumably are not transcribed together. The numbers in parenthesis indicate the approximate length in Kb of each genetic element.

Some Flavr Savr tomato lines transformed with vector pCGN1436 are:
501-1436-1001, 502-1436-2021, 7B-1436-92, 22B-1436-215, 28B-1436-419, 28B-1436-425, 28B-1436-498, 501-1436-1035, 105F-1436-2018, 105F-1436-2035, 105F-1436-2049 and N73-1436-111.

Some Flavr Savr tomato lines transformed with vector pCGN4109 are:
35F-4109a-3023, 84F-4109a-148, 88F-4109a-2797, 121F-4109a-333, 121F-4109a-1071, 121F-4109a-1120, 137F-4109a-71, 138F-4109a-164, 519A-4109a-4527, 519A-4109a-4621, 519A-4109a-4676, 531A-4109a-2105, 531A-4109a-2270, 532A-4109a-5097, 585A-4109a-3604, 585A-4109a-3530, 540A-4109a-1739, 532A-4109a-5166, 519A-4109a-4645, 540A-4109a-1823, 114F-4109a-26 and 114F-4109a-81.
LMO characteristics
Modified traits
Other gene(s) whose expression was affected by the transformation
Polygalacturonase gene - Solanum lycopersicum - Tomato, SOLLC
Changes in physiology and/or production - Ripening
How the expression of the gene(s) was affected
As a result of the introduction of a pg gene in the anti-sense orientation, the expression of the endogenous pg gene was suppressed.

The expression of other genes may have been affected in FLAVR SAVR™ tomato as a result of the transformation. 
Common use(s)
  • Food

Records referencing this document (11)
IDDescription
11record(s) found
Country's Decision or any other Communication9 records
Organization1 record
Risk Assessment1 record