Notes about the Other(s) sequence(s) specific to this
The pg gene from tomato was introduced in the anti-sense
orientation to suppress the expression of the endogenous
Depending on the construct/vector used (see below), the antisense
pg gene was under regulatory control of a single or two
tandem copies of the 35S promoter from the cauliflower mosaic virus
(CaMV). The terminator sequences for the antisense pg gene
were the tml gene (T-tml) and the transcript 7 gene from
the octopine-type Ti plasmid pTiA6 from A. tumefaciens.
Expression of the nptII gene was under the control of the
5' promoter and 3' terminator sequences from the mannopine synthase
gene derived from A. tumefaciens or the 35s promoter and
T-tml terminator, depending on the construct introduced in each
The DNA cassettes in two of the vectors used are shown below.
Information on the cassettes in other vectors used to modify FLAVR
SAVR™ was not found.
DNA cassette in pCGN1436:
LB (0.58) | P-mas (0.68) >> nptII (0.98) >>
T-mas (0.77) | lac (0.29) | P-35S (0.6) >> P-35S
(0.6) << pg (1.6) << Space (0.012) >>
T-tml (1.2) | lac (0.17) | RB (0.28)
DNA cassette in pCGN4109:
LB | T-tml << nptII << P-35S | T-tml >>
pg >> P-35S (0.6) << P-35S (0.6) | RB
Note: the symbols >> and << indicate the sense and
anti-sense direction of transcription, respectively, between
different elements (typically, promoter - coding sequence -
terminator). If present, the symbol | indicates elements that may
be cloned adjacently in a cassette but that presumably are not
transcribed together. The numbers in parenthesis indicate the
approximate length in Kb of each genetic element.
Some Flavr Savr tomato lines transformed with vector pCGN1436
501-1436-1001, 502-1436-2021, 7B-1436-92, 22B-1436-215,
28B-1436-419, 28B-1436-425, 28B-1436-498, 501-1436-1035,
105F-1436-2018, 105F-1436-2035, 105F-1436-2049 and N73-1436-111.
Some Flavr Savr tomato lines transformed with vector pCGN4109
35F-4109a-3023, 84F-4109a-148, 88F-4109a-2797, 121F-4109a-333,
121F-4109a-1071, 121F-4109a-1120, 137F-4109a-71, 138F-4109a-164,
519A-4109a-4527, 519A-4109a-4621, 519A-4109a-4676, 531A-4109a-2105,
531A-4109a-2270, 532A-4109a-5097, 585A-4109a-3604, 585A-4109a-3530,
540A-4109a-1739, 532A-4109a-5166, 519A-4109a-4645, 540A-4109a-1823,
114F-4109a-26 and 114F-4109a-81.