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Modified Organism
MON-ØØ531-6 x MON-Ø1445-2 - Roundup Ready™ Bollgard™ Cotton
Record information and status
Record ID
14883
Status
Published
Date of creation
2006-06-05 14:40 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-07-24 13:32 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-24 13:32 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Roundup Ready™ Bollgard™ Cotton
Transformation event
1445 x 531 (MON531 X MON1445)
Unique identifier
MON-ØØ531-6 x MON-Ø1445-2
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
The stacked cotton line MON531 X MON1445 expresses two novel proteins: the delta-endotoxin Cry1Ac which confers resistance to the lepidopteran pests of cotton, such as the cotton bollworm, pink bollworm and tobacco budworm, and the CP4 EPSPS protein which confers tolerance to the herbicide glyphosate. The insecticidal protein is produced by the cry1Ac gene from MON531, and the CP4 EPSPS protein is from the cp4 epsps gene from MON1445. The novel traits of each parental line have been combined, through traditional plant breeding, to produce this new cotton line.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
MON-ØØ531-6 - Bollgard™ cotton
Resistance to antibiotics - Kanamycin, Streptomycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
MON-Ø1445-2 - Roundup Ready™ cotton
Resistance to antibiotics - Kanamycin, Streptomycin Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
PV-GHBK04 and PV-GHGT07
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
FMV 35S promoter
0.57 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.36 Kb
 
 
rbcS-E9 gene terminator
0.63 Kb
 
 
CaMV 35S promoter
0.32 Kb
 
 
Neomycin Phosphotransferase II
0.79 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Cry1Ac
0.00 Kb
 
 
α' subunit of β-conglycinin gene terminator
0.00 Kb
 
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
A stacked insect-resistant and glyphosate-tolerant cotton derived from conventional cross-breeding of MON-ØØ531-6 and MON-Ø1445-2.  Tolerance to the herbicide glyphosate produced through incorporation of the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase (epsps), that confers tolerance to the herbicide glyphosate and resistance to lepidoptera insects from the cry1Ac gene from Bacillus thuringiensis subsp. kurstaki. The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin.

DNA insert from vector PV-GHBK04
From the MON531 parental organism. Contains coding sequences and regulatory elements for cry1A(c) and nptII. The aadA gene was also integrated into the host genome, however it is only expressed in bacterial cells.

DNA insert from vector PV-GHGT07
From the MON1445 parental organism. contains coding sequences and regulatory elements for epsps and nptII. The aadA gene was also integrated into the host genome, however it is only expressed in bacterial cells. The PV-GHGT07 vector also contained a coding sequence for the Glyphosate oxidoreductase (GOX) gene however southern blot analysis indicated that that it was not integrated into the host genome.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Fiber / Textile
Additional Information
Other relevant website address or attached documents

Records referencing this document (37)
IDDescription
37record(s) found
Country's Decision or any other Communication18 records
Organization3 records
Risk Assessment16 records