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Gene and DNA Sequence
Record information and status
Record ID
14973
Status
Published
Date of creation
2006-06-09 15:18 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2014-02-26 20:28 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-02-26 20:28 UTC (dina.abdelhakim@cbd.int)

General information
Name of the gene or DNA sequence
Barnase
Abbreviation
CS-Barnase-BACAS
Category of DNA sequence
  • Protein coding sequence
Is the gene / DNA sequence is a synthetic molecule?
No
Donor organism
Donor organism
Bacillus amyloliquefaciens - BACAS
Characteristics of the protein coding sequence
Name of the protein expressed by the coding sequence
Barnase ribonuclease inhibitor
Biological function
Barnase is a bacterial protein that has ribonuclease activity. It is lethal to the cell when expressed without its inhibitor barstar. The inhibitor binds to and occludes the ribonuclease active site, preventing barnase from damaging the cell's RNA after it has been synthesized but before it has been secreted.

Barnase catalyzes hydrolysis at diribonucleotide GpN sites. Cleavage occurs in two steps using a general acid-base mechanism: a cyclic intermediate is formed during the first transesterification step, which is then hydrolysed to release the cleaved RNA.

When synthesised by plants it causes male sterility by producing a ribonuclease enzyme (RNAse) that interferes with RNA production in specific cells of the pollen sac, thus disrupting their normal cell functioning and preventing the development of pollen which lead to male sterility.
Related trait(s) or use(s) in biotechnology
Additional Information
Other relevant website address or attached documents

Records referencing this document (93)
IDDescription
93record(s) found
Country's Decision or any other Communication48 records
Modified Organism14 records
Organization3 records
Risk Assessment28 records